Difference between revisions of "PM-pathway control state"
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[[Pyruvate]] (P) is oxidatively decarboxylated to acetyl-CoA and CO<sub>2</sub>, yielding [[NADH]] catalyzed by pyruvate dehydrogenase. [[Malate]] (M) is oxidized to oxaloacetate by mt-malate dehydrogenase located in the mitochondrial matrix. Condensation of oxaloacate with acetyl-CoA yields citrate (citrate synthase). 2-oxoglutarate (Ξ±-ketoglutarate) is formed from isocitrate (isocitrate dehydrogenase). | [[Pyruvate]] (P) is oxidatively decarboxylated to acetyl-CoA and CO<sub>2</sub>, yielding [[NADH]] catalyzed by pyruvate dehydrogenase. [[Malate]] (M) is oxidized to oxaloacetate by mt-malate dehydrogenase located in the mitochondrial matrix. Condensation of oxaloacate with acetyl-CoA yields citrate (citrate synthase). 2-oxoglutarate (Ξ±-ketoglutarate) is formed from isocitrate (isocitrate dehydrogenase). | ||
|info=[[Gnaiger | |info=[[Gnaiger 2020 BEC MitoPathways]] | ||
}} | }} | ||
{{MitoPedia concepts | {{MitoPedia concepts | ||
|mitopedia concept=SUIT state | |mitopedia concept=SUIT state | ||
}} | }} | ||
[[File:PM.jpg|right|400px|link=Gnaiger | [[File:PM.jpg|right|400px|link=Gnaiger 2020 BEC MitoPathways |Gnaiger 2020 BEC MitoPathways]] | ||
== PM<sub>''L''</sub> == | == PM<sub>''L''</sub> == | ||
Revision as of 20:12, 1 January 2021
- high-resolution terminology - matching measurements at high-resolution
PM-pathway control state
Description
MitoPathway control state: NADH Electron transfer-pathway state
SUIT protocol: 1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp- - SUIT_RP1
Pyruvate (P) is oxidatively decarboxylated to acetyl-CoA and CO2, yielding NADH catalyzed by pyruvate dehydrogenase. Malate (M) is oxidized to oxaloacetate by mt-malate dehydrogenase located in the mitochondrial matrix. Condensation of oxaloacate with acetyl-CoA yields citrate (citrate synthase). 2-oxoglutarate (Ξ±-ketoglutarate) is formed from isocitrate (isocitrate dehydrogenase).
Abbreviation: PM
Reference: Gnaiger 2020 BEC MitoPathways
MitoPedia concepts:
SUIT state
PML
LEAK state (L) with PM as N-linked substrates can be evaluated in the following SUIT protocols:
-
- DL-Protocol for isolated mitochondria and tissue homogenate (mt): SUIT-001 O2 mt D001
- DL-Protocol for permeabilized fibers (pfi): SUIT-001 O2 pfi D002
- DL-Protocol for permeabilized cells (pce): SUIT-001 O2 ce-pce D003
- DL-Protocol for permeabilized blood cells (PBMC and PLT): SUIT-001 O2 ce-pce D004
- DL-Protocol for permeabilized fibers (pfi): SUIT-004 O2 pfi D010
- DL-Protocol for permeabilized cells (pce): SUIT-006 O2 ce-pce D029
- DL-Protocol for isolated mitochondria and tissue homogenate (mt): SUIT-006 O2 mt D047
- DL-Protocol for permeabilized fibers (pfi): SUIT-008 O2 pfi D014
-
PMP
OXPHOS state (P) with PM as N-linked substrates can be evaluated in the following SUIT protocols:
-
- DL-Protocol for isolated mitochondria and tissue homogenate (mt): SUIT-001 O2 mt D001
- DL-Protocol for permeabilized fibers (pfi): SUIT-001 O2 pfi D002
- DL-Protocol for permeabilized cells (pce): SUIT-001 O2 ce-pce D003
- DL-Protocol for permeabilized blood cells (PBMC and PLT): SUIT-001 O2 ce-pce D004
- DL-Protocol for permeabilized fibers (pfi): SUIT-004 O2 pfi D010
- DL-Protocol for permeabilized cells (pce): SUIT-006 O2 ce-pce D029
- DL-Protocol for isolated mitochondria and tissue homogenate (mt): SUIT-006 O2 mt D047
- DL-Protocol for permeabilized fibers (pfi): SUIT-008 O2 pfi D014
-
PME
ET state (E) with PM as N-linked substrates can be evaluated in the following SUIT protocols:
-
- DL-Protocol for isolated mitochondria and tissue homogenate (mt): SUIT-001 O2 mt D001
- DL-Protocol for permeabilized fibers (pfi): SUIT-001 O2 pfi D002
- DL-Protocol for permeabilized cells (pce): SUIT-001 O2 ce-pce D003
- DL-Protocol for permeabilized blood cells (PBMC and PLT): SUIT-001 O2 ce-pce D004
- DL-Protocol for permeabilized fibers (pfi): SUIT-004 O2 pfi D010
- DL-Protocol for permeabilized cells (pce): SUIT-006 O2 ce-pce D029
- DL-Protocol for isolated mitochondria and tissue homogenate (mt): SUIT-006 O2 mt D047
- DL-Protocol for permeabilized fibers (pfi): SUIT-008 O2 pfi D014
-
Linear coupling control in the N-pathway control state: L β P - E
- L - P
- OXPHOS-coupling efficiency (P-L or βP control factor), jβP = βP/P = (P-L)/P = 1-L/P, is measured in the N-linked pathway state, with defined coupling sites (CI, CIII, CIV) and at high flux.
- P - E
- CCCP is titrated stepwise to maximum flux, to evaluate limitation of OXPHOS by the phosphorylation system, expressed as the apparent excess E-P capacity factor (E-P coupling control factor), jExP = (E-P)/E = 1-P/E. If jExP>0, then the E-L coupling efficiency rather than the OXPHOS-coupling efficiency is the proper expression of coupling, jβE = βE/E = (E-L)/E = 1-L/E.
Discussion
- The Pyruvate anaplerotic pathway control state (pyruvate alone) is not an ET-pathway competent substrate state in most mt-preparations, since acetyl-CoA accumulates without the co-substrate (oxaloacetate) of citrate synthase.
- The Malate-anaplerotic pathway control state (M alone) is not an ET-pathway competent substrate state in many mt-preparations, since oxaloacetate accumulates without the co-substrate (acetyl-CoA) of citrate synthase.
