Park 2010 Sensors and Actuators B
| Park J, Pak YK, Pak JJ (2010) A microfabricated reservoir-type oxygen sensor for measuring the real-time cellular oxygen consumption rate at various conditions. Sensors and Actuators B 147: 263–269. |
Park J, Pak YK, Pak JJ (2010) Sensors and Actuators B: Chemical
Abstract: This paper presents a microfabricated reservoir-type oxygen sensor, which can accurately measure the solubilized oxygen concentration in real time, in order to measure the cellular oxygen consumption rate(OCR) in a solution containing cells. The fabricated oxygen sensor is composed of three-parts: electrochemical This paper presents a microfabricated reservoir-type oxygen sensor, which can accurately measure the solubilized oxygen concentration in real time, in order to measure the cellular oxygen consumption rate (OCR) in a solution containing cells. The fabricated oxygen sensor is composed of three-parts: electrochemical sensing electrodes, an oxygen-permeable membrane, and a reservoir for storing the solution. The oxygen transport rate through the membrane and the oxygen reaction rate at the working electrode (WE) surface are the two dominant parameters in determining the sensitivity of the oxygen sensor. The fabricated sensor showed a sensitivity of 2.84 A/cm2M and a 90% response time of 4.9 s in an average of 5 sensors when a 25,000µm2 WE and a 20µm polydimethylsiloxane membrane were used. This is the first report in which the fastest response time has been achieved for the oxygen sensor. The fabricated sensor showed the repeatability with 154.05±1.87 nA at the full-oxygen state and 2.77±1.0 nA at the zero-oxygen state. The fabricated sensor was used to measure the uncoupled OCR of the L6 cells, and its result of 3.69±0.30 was almost identical to the result of 3.70±0.26 obtained from a commercial system. first report in which the fastest response time has been achieved for the oxygen sensor. The fabricated sensor showed the repeatability with 154.05±1.87 nA at the full-oxygen state and 2.77±1.0 nA at the zero-oxygen state. The fabricated sensor was used to measure the uncoupled OCR of the L6 cells, and its result of 3.69±0.30 was almost identical to the result of 3.70±0.26 obtained from a commercial system. • Keywords: electrochemistry, reservoir-type, oxygen sensor, cellular respiration, oxygen consumption rate
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Organism: Rat
Tissue;cell: Skeletal Muscle"Skeletal Muscle" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.
Preparation: Intact Cell; Cultured; Primary"Intact Cell; Cultured; Primary" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
HRR: Oxygraph-2k
L6 rat skeletal muscle
