Porter 2022 Abstract Bioblast

6.3. «10+5» Karavyraki M, Gnaiger E, Porter Richard K (2022) A comparison of bioenergetics in human tongue pre-cancerous dysplastic oral keratinocytes and squamous cancer cells. Bioblast 2022: BEC Inaugural Conference. In: https://doi.org/10.26124/bec:2022-0001 »MitoFit Preprint«

Link: Bioblast 2022: BEC Inaugural Conference

Karavyraki Marilena, Gnaiger Erich, Porter Richard K (2022)

Event: Bioblast 2022

In an endeavour to understand the metabolic phenotype behind oral squamous cell carcinomas [1], we characterised the bioenergetic profile of a human tongue derived cancer cell line (SCC-4 cells) and compared this profile to a pre-cancerous dysplastic oral keratinocyte (DOK) cell line also derived from human tongue. The human SCC-4 cancer cells had greater mitochondrial density but lower mitochondrial O₂ flow per cell J_O₂ than DOK cells. The lower cell J_O₂ in SCC-4 cells can be partially explained by lower NADH-related enzymatic activity when compared to pre-cancerous DOK cells. In addition, SCC-4 cells have greater extracellular acidification rate (an index of glycolytic flux) when compared to DOK cells. In addition, treatment with recombinant human IL-6 (rhIL-6), known to drive anoikis resistance in SCC-4 cells but not DOK cells, impairs oxygen consumption in SCC-4 but not DOK cells, without affecting mitochondrial density. We conclude that SCC-4 cells have a less oxidative phenotype compared to DOK cells and that IL-6 attenuates mitochondrial function in SCC-4 cells while increasing glycolytic flux.

Karavyraki M, Porter RK (2022) Evidence of a role for interleukin-6 in anoikis resistance in oral squamous cell carcinoma. https://doi.org/10.1007/s12032-022-01664-5

• Keywords: Oral Squamous Cancer Cells, Mitochondria, Interleukin 6, Dysplastic oral keratinocytes, Oxygen consumption • Bioblast editor: Plangger M

Affiliations and support

Marilena Karavyraki¹, Erich Gnaiger², Richard K Porter¹

School of Biochemistry, Trinity Biomedical Science Institute, Trinity College Dublin, Ireland - [email protected]
Oroboros Instruments, Innsbruck, Austria

Acknowledgements: Marie Curie Grant TRACT 721906 H2020-MCSA-ITN 2016; COST Action CA15203 MitoEAGLE (2016-2021).

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Labels: MiParea: Respiration Pathology: Cancer

Organism: Human

Preparation: Permeabilized cells Enzyme: Complex I, Marker enzyme, TCA cycle and matrix dehydrogenases Regulation: Aerobic glycolysis Coupling state: LEAK, ROUTINE, ET Pathway: S, ROX HRR: Oxygraph-2k Event: B2 Crabtree effect