Residual oxygen consumption

high-resolution terminology - matching measurements at high-resolution

Residual oxygen consumption

Description

Residual oxygen consumption, ROX, is the respiration remaining after application of ETS inhibitors to mitochondrial preparations or cells, or in mt-preparations incubated without substrates (in the presence of ADP: State 2).

ROX may be related to, but is different from ROS production.

Abbreviation: ROX

Reference: MiPNet12.15, Gnaiger_2008_POS, Gnaiger_2009_IJBCB

MitoPedia topics: "Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. Respiratory state"Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. 

ROX and non-mitochondrial respiration

• Why 'State 2' but not 'non-mitochondrial respiration'?

Residual oxygen consumption (ROX) is sometimes referred to as 'non-mitochondrial respiration'. This may be correct to a large extent, but is not entirely accurate. In a preparation of purified isolated mitochondria, a small but significant ROX is observed, even after correction for instrumental background oxygen flux. In this case, ROX is 'mitochondrial non-ETS' rather than ‘non-mitochondrial’ respiration. In permeabilized and intact cells, ROX may be higher than in isolated mitochondria, and this increased part then would actually be the non-mitochondrial component of ROX.