Ruthenium red

high-resolution terminology - matching measurements at high-resolution

Ruthenium red

Description

Ruthenium red (synonym: ammoniated ruthenium oxychloride) inhibits the mitochondrial Ca²⁺ uniporter. However, in addition it has been shown to interact with and inhibit a large number of other proteins, including ion channels particularly of the Transient Receptor Potential Vanilloid (TRPV) family [1], Ca²⁺-ATPases, and, importantly, the voltage-dependent anion channel (VDAC) [2].

Abbreviation: RR

Reference: MiPNet03.02, Wikipedia

Application in HRR

RR: Ruthenium red (Ammoniated ruthenium oxychloride; (NH₃)₅RuORu(NH₃)₄ORu(NH₃)₅Cl₆), Sigma-Aldrich: R2751, store at RT, CAS: 11103-72-3, M = 786.35 g·mol^-1

Hazard statements: H302; Harmful if swallowed

Preparation of 2 mM stock solution (dissolved in H₂O) for 2-mL O2k-chamber:

1. Weigh 1.57 mg Ruthenium Red.
2. Dissolve in 1 mL H₂O.
3. Divide into 0.1 mL aliquots.
4. Store at -20 °C.

» O2k manual titrations MiPNet09.12 O2k-Titrations

• Titration volume (2-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
• Final concentration: 1 µM.

Preparation of 0.5 mM stock solution (dissolved in H₂O) for 0.5-mL O2k-chamber:

1. Take 50 µL of the 1 mM stock solution.
2. Add 150 µL H₂O.

» O2k manual titrations MiPNet09.12 O2k-Titrations

• Titration volume (0.5-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
• Final concentration: 1 µM.

References

1. Amann R, Maggi CA (1991) Ruthenium red as a capsaicin antagonist.Life Sci. 9: 849-856.
2. Israelson A, Zaid H, Abu-Hamad S, Nahon E, Shoshan-Barmatz V (2008) Mapping the ruthenium red-binding site of the voltage-dependent anion channel-1. Cell Calcium 43:196-204.

MitoPedia topics: Inhibitor