SUIT-003 O2 ce D028: Difference between revisions
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|description=[[File:Ce R 1Omy 2U 3Rot 4S 5Ama.jpg|right|200px]] | |description=[[File:Ce R 1Omy 2U 3Rot 4S 5Ama.jpg|right|200px]] | ||
'''[[Categories of SUIT protocols |SUIT-catg]]:''' CC+S | '''[[Categories of SUIT protocols |SUIT-catg]]:''' CC+S | ||
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|mitopedia concept=SUIT protocol | |mitopedia concept=SUIT protocol | ||
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::: '''[[SUIT protocol pattern]]:''' linear [[coupling control protocol]] (CCP) | |||
::: '''[[Mark names in DatLab]]:''' CCP_R1 | |||
::: '''[[DatLab-Excel templates |DatLab-Excel template]]:''' ''in prep.'': CCP_R1.xlsx | |||
:::* '''3Rot 4S 5Ama''' | :::* '''3Rot 4S 5Ama''' | ||
Revision as of 14:15, 29 August 2016
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 O2 ce D028
Description
SUIT-catg: CC+S
Abbreviation: CC_R 1Omy 2U 3Rot 4S 5Ama
MitoPedia concepts:
SUIT protocol
- SUIT protocol pattern: linear coupling control protocol (CCP)
- Mark names in DatLab: CCP_R1
- DatLab-Excel template: in prep.: CCP_R1.xlsx
- 3Rot 4S 5Ama
- 3Rot: In many intact cells ROX measured after titration of rotenone (3Rot) is not or only slightly further inhibited by subsequent titration of animycin A (CCP Huetter 2004 Biochem J; compare CCP MiPNet08.09 CellRespiration, Ce_R 1Omy 2U 3Rot).
- 4S: Succinate is not permeable through the plasma membrane of many intact cells (Steinlechner-Maran 1997 Transplantation). If succinate is accessible to rotenone-inhibited uncoupled mitochondria, respiration will be stimulated.
- 5Ama: Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration (Stadlmann_2006_Cell Biochem Biophys).
