SUIT-003 O2 ce D028: Difference between revisions
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::: '''[[Categories of SUIT protocols |SUIT-catg]]:''' CCP(S) | ::: '''[[Categories of SUIT protocols |SUIT-catg]]:''' CCP(S) | ||
::: '''[[SUIT protocol pattern]]:''' linear [[coupling control protocol]] (CCP) | ::: '''[[SUIT protocol pattern]]:''' linear [[coupling control protocol]] (CCP) | ||
::: '''[[Mark names in DatLab]]:''' CCP(S) | ::: '''[[Mark names in DatLab]]:''' CCP(S)R2 | ||
::: '''[[DatLab-Excel templates |DatLab-Excel template]]:''' ''in prep.'': CCP(S) | ::: '''[[DatLab-Excel templates |DatLab-Excel template]]:''' ''in prep.'': CCP(S)R2.xlsx | ||
:::* '''3Rot 4S 5Ama''' | :::* '''3Rot 4S 5Ama''' | ||
:::: '''3Rot''': In many intact cells ROX measured after titration of rotenone (3Rot) is not or only slightly further inhibited by subsequent titration of animycin A ([[ | :::: '''3Rot''': In many intact cells ROX measured after titration of rotenone (3Rot) is not or only slightly further inhibited by subsequent titration of animycin A ([[CCP_02|CCP_02a]]. | ||
:::: '''4S''': Succinate is not permeable through the plasma membrane of many intact cells ([[Steinlechner-Maran 1997 Transplantation]]). If succinate is accessible to rotenone-inhibited uncoupled mitochondria, respiration will be stimulated. | :::: '''4S''': Succinate is not permeable through the plasma membrane of many intact cells ([[Steinlechner-Maran 1997 Transplantation]]). If succinate is accessible to rotenone-inhibited uncoupled mitochondria, respiration will be stimulated. | ||
:::: '''5Ama''': Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration ([[Stadlmann_2006_Cell Biochem Biophys]]). | :::: '''5Ama''': Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration ([[Stadlmann_2006_Cell Biochem Biophys]]). | ||
Revision as of 13:27, 3 September 2016
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 O2 ce D028
Description
Abbreviation: CCP_R 1Omy 2U 3Rot 4S 5Ama
MitoPedia concepts:
SUIT protocol
- SUIT-catg: CCP(S)
- SUIT protocol pattern: linear coupling control protocol (CCP)
- Mark names in DatLab: CCP(S)R2
- DatLab-Excel template: in prep.: CCP(S)R2.xlsx
- 3Rot 4S 5Ama
- 3Rot: In many intact cells ROX measured after titration of rotenone (3Rot) is not or only slightly further inhibited by subsequent titration of animycin A (CCP_02a.
- 4S: Succinate is not permeable through the plasma membrane of many intact cells (Steinlechner-Maran 1997 Transplantation). If succinate is accessible to rotenone-inhibited uncoupled mitochondria, respiration will be stimulated.
- 5Ama: Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration (Stadlmann_2006_Cell Biochem Biophys).
