SUIT-005 O2 pfi D011: Difference between revisions

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::: '''[[SUIT protocol pattern]]:''' diametral
::: '''[[SUIT protocol pattern]]:''' diametral


::: '''[[Laboratory titration sheet]]:''' [[Media:TargetRP2_FNS(PM)01_D(c)_pfi.pdf |Β»SUIT TargetRP2_FNS(PM)01_D(c)_pfi]]
::: '''[[DatLab-Analysis templates |DatLab-Analysis template]]:''' ''in prep.'' SUIT_FNS(PM)01.xlsx
::::* Linked to [[1D;2OctM;3P;4G;5S;6Gp;7U;8Rot-]] - SUIT RP2, specifically for human skeletal muscle mitochondria.
::::* Linked to [[1D;2OctM;3P;4G;5S;6Gp;7U;8Rot-]] - SUIT RP2, specifically for human skeletal muscle mitochondria.
::::* Harmonized with [[1PM;2D;3U;4S;5Rot-]].
::::* Harmonized with [[1PM;2D;3U;4S;5Rot-]].
__TOC__




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::::* [[Isolated mitochondria |imt]], ROX: 1OctM;2D;2c;3P;4S;5U;6Rot;7Ama
::::* [[Isolated mitochondria |imt]], ROX: 1OctM;2D;2c;3P;4S;5U;6Rot;7Ama
::::* [[Permeabilized muscle fibres |pfi]], high O<sub>2</sub>, no ROX: FNS(PM)01_pfiO2,1OctM,2D(c),3P,4S,5U,6Rot
::::* [[Permeabilized muscle fibres |pfi]], high O<sub>2</sub>, no ROX: FNS(PM)01_pfiO2,1OctM,2D(c),3P,4S,5U,6Rot
== SUIT O2 DLP for RP2m (DatLab 7.1) ==
:::: [[DL-Protocols]] (DLP) for substrate-uncoupler-inhibitor titrations (see [[MitoPedia: SUIT]]) guide through a sequence of coupling and pathway control states. A specific SUIT protocol can be assigned to O2k-Chamber A or B or both. A list of DL-protocols is displayed in the [[DL-Protocols#DL-Protocol_library| DL-Protocol library]].
'''Download instructions'''.<br/>To start the download click on the [[File:Download1.png|15px]] icon. Depending on your browser you will encounter different download scenarios.<br/>(i) A window opens to select whether to "open file with" or to "save file". Please always choose the option "save file"; opening the file directly will not work due to safety reasons.<br/>(ii) When you click the download icon, some browsers will display the text/content of the .DLP file instead of downloading it. In this case go back to this download page and '''right-click''' the download icon and choose "save linked file as" from the context-menu. A window will open to save the file. In some cases the suffix of the file might be wrong (e.g. ".HTML") - you must correct the suffix of the file to ".DLP".
{| class="wikitable" border="1"
|-
! DL-Protocol
! Description
! Download - Protocol .DLP
! Download - Analysis .XLSX
|-
| RP2m
| SUIT reference protocol RP2 modified for permeabilized fibers
| style="text-align:center"| [[File:Download1.png|20px|link=http://wiki.oroboros.at/images/d/d6/RP2m_pfi.DLP]]
| [[1OctM;2D;3P;4S;5U;6Rot-|1OctM;2D;3P;4S;5U;6Rot-]]
| style="text-align:center"| [[File:Download2.png|20px|link=http://wiki.oroboros.at/images/f/fc/RP2m_pfi.xlsx]]
|}

Revision as of 16:44, 6 September 2017


high-resolution terminology - matching measurements at high-resolution


SUIT-005 O2 pfi D011

Description

1OctM;2D;3P;4S;5U;6Rot-.jpg

Abbreviation: FNS(PM)

Reference: A: RP2m (RP2 modified) is a short SUIT protocol linked to 1D;2OctM;3P;4G;5S;6Gp;7U;8Rot- - SUIT RP2 (human skeletal muscle)


MitoPedia concepts: SUIT protocol, SUIT A 

SUIT-category: FNS(PM)
SUIT protocol pattern: diametral



1OctM;2D;2c;3P;4S;5U;6Rot-

SUIT FNS(PM)01 D(c).jpg 1OctM;2D;2c;3P;4S;5U;6Rot-

Specific cases
  • imt, ROX: 1OctM;2D;2c;3P;4S;5U;6Rot;7Ama
  • pfi, high O2, no ROX: FNS(PM)01_pfiO2,1OctM,2D(c),3P,4S,5U,6Rot


SUIT O2 DLP for RP2m (DatLab 7.1)

DL-Protocols (DLP) for substrate-uncoupler-inhibitor titrations (see MitoPedia: SUIT) guide through a sequence of coupling and pathway control states. A specific SUIT protocol can be assigned to O2k-Chamber A or B or both. A list of DL-protocols is displayed in the DL-Protocol library.


Download instructions.
To start the download click on the Download1.png icon. Depending on your browser you will encounter different download scenarios.
(i) A window opens to select whether to "open file with" or to "save file". Please always choose the option "save file"; opening the file directly will not work due to safety reasons.
(ii) When you click the download icon, some browsers will display the text/content of the .DLP file instead of downloading it. In this case go back to this download page and right-click the download icon and choose "save linked file as" from the context-menu. A window will open to save the file. In some cases the suffix of the file might be wrong (e.g. ".HTML") - you must correct the suffix of the file to ".DLP".


DL-Protocol Description Download - Protocol .DLP Download - Analysis .XLSX
RP2m SUIT reference protocol RP2 modified for permeabilized fibers Download1.png 1OctM;2D;3P;4S;5U;6Rot- Download2.png
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