Difference between revisions of "SUIT-009 O2 ce-pce D016"
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:::* Cytochrome c test can be performed in the S-OXPHOS state. | :::* Cytochrome c test can be performed in the S-OXPHOS state. | ||
:::* Reasonable duration of the experiment. | :::* Reasonable duration of the experiment. | ||
::: +This protocol can be extended with the Complex IV module. | |||
:::- In some cell lines e.g. HEK293T, P does not have any effect because of the downregulation of the N-linked pathway. | :::- In some cell lines e.g. HEK293T, P does not have any effect because of the downregulation of the N-linked pathway. | ||
Revision as of 16:43, 21 January 2019
- high-resolution terminology - matching measurements at high-resolution
SUIT-009 O2 ce-pce D016
Description
Abbreviation: NS(P)
Reference: A: SUIT-009 short protocol for mt-respiration in pce.
SUIT number: D016_ce1;1Dig;1S;2D;3P;4Rot;5Ama
- SUIT-category: NS(P)
- SUIT protocol pattern: orthogonal 1S;2D;3P;4Rot;5Ama
SUIT protocols
References
Strengths and limitations
- For H2O2-Application, choose SUIT-009 AmR pce D019; for O2 and H2O2 measurements (AmR), choose: SUIT-009 AmR pce D019.
- Cytochrome c test can be performed in the S-OXPHOS state.
- Reasonable duration of the experiment.
- +This protocol can be extended with the Complex IV module.
- - In some cell lines e.g. HEK293T, P does not have any effect because of the downregulation of the N-linked pathway.
MitoPedia concepts: SUIT protocol, SUIT A, Find
MitoPedia methods:
Respirometry
