Difference between revisions of "SUIT-009 O2 ce-pce D016"
From Bioblast
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::: '''[[SUIT protocol pattern]]:''' orthogonal 1S;2D;3P;4Rot;5Ama | ::: '''[[SUIT protocol pattern]]:''' orthogonal 1S;2D;3P;4Rot;5Ama | ||
__TOC__ | __TOC__ | ||
Communicated by [[Iglesias-Gonzalez J], [[Komlodi T]] and [[Gnaiger E]] (last update 2019-01-22) | Communicated by [[Iglesias-Gonzalez J]], [[Komlodi T]] and [[Gnaiger E]] (last update 2019-01-22) | ||
== SUIT protocols == | == SUIT protocols == | ||
::::* [[SUIT-009 AmR mt D021]] | ::::* [[SUIT-009 AmR mt D021]] | ||
Revision as of 17:18, 22 January 2019
- high-resolution terminology - matching measurements at high-resolution
SUIT-009 O2 ce-pce D016
Description
Abbreviation: NS(P)
Reference: A: SUIT-009 short protocol for mt-respiration in pce.
SUIT number: D016_ce1;1Dig;1S;2D;3P;4Rot;5Ama
- SUIT-category: NS(P)
- SUIT protocol pattern: orthogonal 1S;2D;3P;4Rot;5Ama
Communicated by Iglesias-Gonzalez J, Komlodi T and Gnaiger E (last update 2019-01-22)
SUIT protocols
References
Strengths and limitations
- For H2O2-Application, choose SUIT-009 AmR pce D019; for O2 and H2O2 measurements (AmR), choose: SUIT-009 AmR pce D019.
- Cytochrome c test can be performed in the S-OXPHOS state.
- Reasonable duration of the experiment.
- +This protocol can be extended with the Complex IV module.
- - In some cell lines e.g. HEK293T, P does not have any effect because of the downregulation of the N-linked pathway.
MitoPedia concepts: SUIT protocol, SUIT A, Find
MitoPedia methods:
Respirometry
