SUIT-009 O2 ce-pce D016

high-resolution terminology - matching measurements at high-resolution

SUIT-009 O2 ce-pce D016

Description

Abbreviation: NS(P)

Reference: A: SUIT-009 short protocol for mt-respiration in pce.

SUIT number: D016_ce1;1Dig;1S;2D;3P;4Rot;5Ama

SUIT-category: NS(P)

SUIT protocol pattern: orthogonal 1S;2D;3P;4Rot;5Ama

Communicated by Iglesias-Gonzalez J,  Komlodi T and Gnaiger E (last update 2019-01-22)

SUIT protocols

• SUIT-009 AmR mt D021
• SUIT-009 AmR pce D019
• SUIT-009 O2 mt D015
• SUIT-009 O2 pce D016

Strengths and limitations

• For H₂O₂-Application, choose SUIT-009 AmR pce D019; for O2 and H₂O₂ measurements (AmR), choose: SUIT-009 AmR pce D019.
• Cytochrome c test can be performed in the S-OXPHOS state.
• Reasonable duration of the experiment.

+This protocol can be extended with the Complex IV module.

- In some cell lines e.g. HEK293T, P does not have any effect because of the downregulation of the N-linked pathway.

MitoPedia concepts: SUIT protocol, SUIT A, Find 

MitoPedia methods: Respirometry