- high-resolution terminology - matching measurements at high-resolution
SUIT-009 O2 mt D015
Description
Abbreviation: NS(P)
Reference: A: SUIT-009 short protocol for respiration in mitochondrial preparations (isolated mitochondria and tissue homogenate).
SUIT number: D015_1S;2D;3P;4Rot;5Ama
O2k-Application: O2
- SUIT-category: NS(P)
- SUIT protocol pattern: orthogonal 1S;2D;3P;4Rot
1S;2D;3P;4Rot;5Ama-
SUIT-009 is a short protocol for determination of O2 flux on isolated mitochondria,tissue homogenate and permeabilized cells. Succinate (S) supports the reverse electron transfer (RET) initiated H2O2 production in the LEAK state. Addition of ADP increases S-OXPHOS capacity. Pyruvate (P) supports NADH-pathway (N). Antimycin A (Ama) inhibits Complex III (CIII) and results in the inhibition of O2 flux. For H2O2-Application, choose SUIT-009 AmR mt D021; for O2 and H2O2 measurements (AmR), choose: SUIT-009 AmR mt D021.
MitoPedia concepts:
SUIT protocol,
SUIT A,
Find
MitoPedia methods:
Respirometry
Strengths and limitations
- Cytochrome C test can be performed in the S-OXPHOS state.
- +Reasonable duration of the experiment.
