From Bioblast
- high-resolution terminology - matching measurements at high-resolution
SUIT-011
Description
SUIT-catg: NS
Abbreviation: NS_1GM 2D 3c 4S 5U 6Rot 7Ama
Reference: MiPNet12.23 FibreRespiration
MitoPedia concepts:
SUIT protocol
- Subcatg: N+NS+S
- SUIT protocol pattern: diametral
- Mark names in DatLab: MiPNet12_23_FibreRespiration
- DatLab-Excel template: SUIT_MiPNet12.23_FibreRespiration.xlsx
| Step | Respiratory state | Pathway control | Pathway to Q | Comment |
|---|---|---|---|---|
| 1GM | GM(L) | N | CI | See 2D.
|
| 2D | GM(P) | N | CI | GM and PM yield practically identical fluxes in human skeletal muscle fibres. However, PM is the superior alternative to GM, since the fractions of the N-pathway is lower and of the S-pathway is higher with GM compared to PM. PM, therefore, yields a more sensitive assay for the diagnosis of injuries in the N-linked (CI-linked) pathway. Compare SUIT targetRP1 NS and SUIT RP1. |
| 3c | GM(c) | N | CI | Cytochrome c added immediately after the earliest ADP-activation step. Application of the cytochrome c test early in the protocol ensures comparability of all states in case of any effect of c (Gnaiger 2007 MitoPathways). |
| 3S | GMS(P) | NS | CI&II | |
| 5U | GMS(E) | NS | CI&II | The additive effect of N+S measured separately compared to the combined NS pathway cannot be evaluated in the same coupling state. With NP, NSP, NSE, and SE. If independent information is available on SP = SE, the additivity can be calculated for the OXPHOS state. |
| 6Rot | S(E) | S | CII | A succinate concentration of >10 mM may be required for saturating SE capacity. |
| 10Ama | ROX | ROX | ROX may be lower in substrate states earlier in the SUIT protocol. Therefore, this ROX measurement is frequently taken as a methodological control rather than as the basis of ROX correction of mitochondrial respiration (mt). |
