Difference between revisions of "SUIT-015 O2 pti D043"
From Bioblast
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::: '''[[SUIT protocol pattern]]:''' diametral 1OctM;2D;3G;4P;5S;6U:7Rot- | ::: '''[[SUIT protocol pattern]]:''' diametral 1OctM;2D;3G;4P;5S;6U:7Rot- | ||
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{{Template:SUIT-015 O2 pti D042}} | {{Template:SUIT-015 O2 pti D042}} | ||
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::::* [[SUIT-019]] | ::::* [[SUIT-019]] | ||
::::* [[1PGM;2D;3S;4U;5Rot-]] 1PGM;2D;3S;4U;5Rot- | ::::* [[1PGM;2D;3S;4U;5Rot-]] 1PGM;2D;3S;4U;5Rot- | ||
== References == | |||
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Revision as of 11:39, 14 February 2019
- high-resolution terminology - matching measurements at high-resolution
SUIT-015 O2 pti D043
Description
Abbreviation: FNS(Oct,PGM)
Reference: A pti: permeabilized tissue- SUIT-015
SUIT number: D042_1OctM;2D;3G;4P;5S;6U;7Rot;8Ama
O2k-Application: O2
- SUIT-category: FNS(Oct,PGM)
- SUIT protocol pattern: diametral 1OctM;2D;3G;4P;5S;6U:7Rot-
Strengths and limitations
- + The protocol provides information on FAO capacity in the absence of other, potentially interfering pathways, both in the LEAK state and in OXPHOS.
- + FNS OXPHOS capacity comprises the most important pathways in many cell types and thus provides a physiologically relevant estimate of maximum mitochondrial respiratory capacity.
- + FNS ET capacity is a good estimate of overall ET capacity in many cell types.
- + The presence of PMG and S establishes a fully operative TCA cycle activity.
- + Application of the cytochrome c test early in the protocol ensures comparability of all states in case of any effect of c.
- + Reasonable duration of the experiment.
- - F OXPHOS capacity may be underestimated. In human heart muscle addition of Oct to palmitoylcarnitine (Pal) + malate increased OXPHOS by 26% (Lemuieux et al 2011).
- - SRot(E) may be underestimated if S is not saturating.
- - CIV activity is not measured, to save experimental time.
Compare SUIT protocols
- SUIT-002
- SUIT-019
- 1PGM;2D;3S;4U;5Rot- 1PGM;2D;3S;4U;5Rot-
References
MitoPedia concepts: SUIT protocol, SUIT A, Find
MitoPedia methods:
Respirometry
