SUIT protocol library: Difference between revisions
From Bioblast
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=== GM+D+P+S+F+Rot === | === GM+D+P+S+F+Rot === | ||
# ''Innsbruck protocol 1'': GM+D+c+P+S+F+Rot: | # ''Innsbruck protocol 1'': GM+D+c+P+S+F+Rot: | ||
* Respiratory states: CI(L) - CI(P) - CI+II(P) - CI+II(E) - CII(E) - ROX | * Respiratory states: CI(L) - CI(P) - CI+II(P) - CI+II(E) - CII(E) - ROX | ||
* [[Cytochrome c|''Cyt c'']]: early addition of ''c'' ensures comparability of all states in case of any ''c'' effect | |||
* ([[Gnaiger_2007_MitoPathways]]) | * ([[Gnaiger_2007_MitoPathways]]) | ||
Revision as of 13:55, 29 September 2011
CI
CII
CI+CII
CI - CI+CII - CII
GM+D+S+F+Rot
- GM+D+c+S+F+Rot: cyt c added after ADP (Boushel 2007 Diabetologia)
- GM+D+S+c+F+Rot: cyt c added after S
- ADP conc:
- ROX: Ama (Boushel 2007 Diabetologia)
GM+D+P+S+F+Rot
- Innsbruck protocol 1: GM+D+c+P+S+F+Rot:
- Respiratory states: CI(L) - CI(P) - CI+II(P) - CI+II(E) - CII(E) - ROX
- Cyt c: early addition of c ensures comparability of all states in case of any c effect
- (Gnaiger_2007_MitoPathways)
PM+D+G+S+F+Rot
- Innsbruck protocol 2: PM+D+c+G+S+F+Rot
- Respiratory states: CI(L) - CI(P) - CI+II(P) - CI+II(E) - CII(E) - ROX
- (Gnaiger_2007_MitoPathways)
PMG+D+S+F+Rot
- PMG+D+c+S+F+Rot
- Respiratory states: CI(L) - CI(P) - CI+II(P) - CI+II(E) - CII(E)
- ADP conc: in mouse brain homogenate a final conc. of 2 mM ADP in the OXPHOS state (CI+CII) is saturating (Sumbalova_2011_Abstract)
- ROX: Mna+Ama; Myx (Gnaiger_2009_IJBCB)
