Difference between revisions of "Salicylhydroxamic acid"
From Bioblast
(replaced FW by M and added units g*mol-1) |
|||
| Line 7: | Line 7: | ||
== Application in [[HRR]] == | == Application in [[HRR]] == | ||
::: '''SHAM: Salicylhydroxamic acid''' (99%; HOC<sub>6</sub>H<sub>4</sub>CONHOH); Sigma S 607, 5 g, store at RT; | ::: '''SHAM: Salicylhydroxamic acid''' (99%; HOC<sub>6</sub>H<sub>4</sub>CONHOH); Sigma S 607, 5 g, store at RT; M = 153.14 g·mol<sup>-1</sup>. | ||
::::<span style="color:#8B008B"> '''Caution:''' Irritant!</span> | ::::<span style="color:#8B008B"> '''Caution:''' Irritant!</span> | ||
Revision as of 13:06, 12 February 2021
- high-resolution terminology - matching measurements at high-resolution
Salicylhydroxamic acid
Description
Salicylhydroxamic acid (SHAM; synonym: 2-Hydroxybenzohydroxamic acid N,2-Dihydroxybenzamide) is an inhibitor of the alternative oxidase (AOX). When AOX is blocked by SHAM, electrons are forced through the CIII-cytochrome c oxidase pathway, allowing observation of the operation of the CIII-CIV pathway without AOX activity.
Abbreviation: SHAM
Reference: Parrino_2000_J_Exp_Biol
Application in HRR
- SHAM: Salicylhydroxamic acid (99%; HOC6H4CONHOH); Sigma S 607, 5 g, store at RT; M = 153.14 g·mol-1.
- Caution: Irritant!
- Preparation of 20 mM stock solution (dissolved in DMSO):
- Weigh 6.1 mg of SHAM.
- Dissolve in 2 mL of DMSO.
- Divide into 0.2 mL portions
- Store at -20 °C.
- Preparation of 20 mM stock solution (dissolved in DMSO):
- » O2k Manual Titrations: MiPNet09.12 O2k-Titrations
- Titration volume: 100 µL
- Final conc. in 2 mL O2k chamber: 1 mM
MitoPedia topics:
Inhibitor
