Salicylhydroxamic acid: Difference between revisions
From Bioblast
(replaced FW by M and added units g*mol-1) |
(added instructions for preparation of higher concentration plus hint, that EtOH could be used, too) |
||
| Line 8: | Line 8: | ||
::: '''SHAM: Salicylhydroxamic acid''' (99%; HOC<sub>6</sub>H<sub>4</sub>CONHOH); Sigma S 607, 5 g, store at RT; M = 153.14 g·mol<sup>-1</sup>. | ::: '''SHAM: Salicylhydroxamic acid''' (99%; HOC<sub>6</sub>H<sub>4</sub>CONHOH); Sigma S 607, 5 g, store at RT; M = 153.14 g·mol<sup>-1</sup>. | ||
::: SHAM is reported to be easily soluble in EtOH. So EtOH could be considered as a solvent, too. However, in Oroboros-lab EtOH has not been tested as a solvent yet. | |||
::::<span style="color:#8B008B"> '''Caution:''' Irritant!</span> | ::::<span style="color:#8B008B"> '''Caution:''' Irritant!</span> | ||
::::'''Preparation of | ::::'''Preparation of 250 mM stock solution (dissolved in DMSO)''': | ||
::::# Weigh | ::::# Weigh 76.57 mg of SHAM. | ||
::::# Dissolve in | ::::# Dissolve in 1950 µL of DMSO. | ||
::::# Divide into 0.2 mL portions | ::::# Divide into 0.2 mL portions | ||
::::# Store at -20 °C. | ::::# Store at -20 °C. | ||
| Line 19: | Line 21: | ||
:::» '''O2k Manual Titrations:''' [[MiPNet09.12 O2k-Titrations]] | :::» '''O2k Manual Titrations:''' [[MiPNet09.12 O2k-Titrations]] | ||
::::* Titration volume: | ::::* Titration volume: 8 µL | ||
::::* Final conc. in 2 mL O2k chamber: 1 mM | ::::* Final conc. in 2 mL O2k chamber: 1 mM | ||
Revision as of 13:09, 3 March 2021
- high-resolution terminology - matching measurements at high-resolution
Salicylhydroxamic acid
Description
Salicylhydroxamic acid (SHAM; synonym: 2-Hydroxybenzohydroxamic acid N,2-Dihydroxybenzamide) is an inhibitor of the alternative oxidase (AOX). When AOX is blocked by SHAM, electrons are forced through the CIII-cytochrome c oxidase pathway, allowing observation of the operation of the CIII-CIV pathway without AOX activity.
Abbreviation: SHAM
Reference: Parrino_2000_J_Exp_Biol
Application in HRR
- SHAM: Salicylhydroxamic acid (99%; HOC6H4CONHOH); Sigma S 607, 5 g, store at RT; M = 153.14 g·mol-1.
- SHAM is reported to be easily soluble in EtOH. So EtOH could be considered as a solvent, too. However, in Oroboros-lab EtOH has not been tested as a solvent yet.
- Caution: Irritant!
- Preparation of 250 mM stock solution (dissolved in DMSO):
- Weigh 76.57 mg of SHAM.
- Dissolve in 1950 µL of DMSO.
- Divide into 0.2 mL portions
- Store at -20 °C.
- Preparation of 250 mM stock solution (dissolved in DMSO):
- » O2k Manual Titrations: MiPNet09.12 O2k-Titrations
- Titration volume: 8 µL
- Final conc. in 2 mL O2k chamber: 1 mM
MitoPedia topics:
Inhibitor
