Difference between revisions of "Salicylhydroxamic acid"
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
{{Chemical_description | |||
|abbr=SHAM | |||
|trivial name=Salicylhydroxamic acid | |||
|complete name=2-Hydroxybenzohydroxamic acid | |||
|chem formula=HOC<sub>6</sub>H<sub>4</sub>CONHOH | |||
|molar mass=153.14 | |||
|vendor=Sigma-Aldrich | |||
|product number=S607 | |||
|store at=RT | |||
|sensitivity= | |||
|cas=89-73-6 | |||
|h statements=H312, H332, H315, H319, H335, H351 | |||
|h info= | |||
harmful in contact with skin, harmful if inhaled, causes skin irritation, causes serious eye irritation, may cause respiratory irritation, suspected of causing cancer}}<!-- ::: '''SHAM: Salicylhydroxamic acid''' (99%; HOC<sub>6</sub>H<sub>4</sub>CONHOH); Sigma S 607, 5 g, store at RT; M = 153.14 g·mol<sup>-1</sup>. | |||
::::<span style="color:#8B008B"> '''Caution:''' Irritant!</span>--> | |||
:::: SHAM is reported to be easily soluble in EtOH. So EtOH could be considered as a solvent, too. However, in Oroboros-lab SHAM in EtOH has not been tested yet. | :::: SHAM is reported to be easily soluble in EtOH. So EtOH could be considered as a solvent, too. However, in Oroboros-lab SHAM in EtOH has not been tested yet. | ||
::::'''Preparation of 250 mM stock solution (dissolved in DMSO)''': | ::::'''Preparation of 250 mM stock solution (dissolved in DMSO)''': | ||
Revision as of 11:59, 11 June 2021
- high-resolution terminology - matching measurements at high-resolution
Salicylhydroxamic acid
Description
Salicylhydroxamic acid (SHAM; synonym: 2-Hydroxybenzohydroxamic acid N,2-Dihydroxybenzamide) is an inhibitor of the alternative oxidase (AOX). When AOX is blocked by SHAM, electrons are forced through the CIII-cytochrome c oxidase pathway, allowing observation of the operation of the CIII-CIV pathway without AOX activity.
Abbreviation: SHAM
Reference: Parrino_2000_J_Exp_Biol
Application in HRR
- SHAM: Salicylhydroxamic acid (2-Hydroxybenzohydroxamic acid; HOC6H4CONHOH), Sigma-Aldrich: S607, store at RT, CAS: 89-73-6, M = 153.14 g·mol-1
- Hazard statements: H312, H332, H315, H319, H335, H351; harmful in contact with skin, harmful if inhaled, causes skin irritation, causes serious eye irritation, may cause respiratory irritation, suspected of causing cancer
- SHAM: Salicylhydroxamic acid (2-Hydroxybenzohydroxamic acid; HOC6H4CONHOH), Sigma-Aldrich: S607, store at RT, CAS: 89-73-6, M = 153.14 g·mol-1
- SHAM is reported to be easily soluble in EtOH. So EtOH could be considered as a solvent, too. However, in Oroboros-lab SHAM in EtOH has not been tested yet.
- Preparation of 250 mM stock solution (dissolved in DMSO):
- Weigh 76.57 mg of SHAM.
- Dissolve in 1950 µL of DMSO.
- Divide into 0.2 mL portions
- Store at -20 °C.
- Preparation of 250 mM stock solution (dissolved in DMSO):
- » O2k Manual Titrations: MiPNet09.12 O2k-Titrations
- Titration volume: 8 µL
- Final conc. in 2 mL O2k chamber: 1 mM
MitoPedia topics:
Inhibitor
