Difference between revisions of "Salicylhydroxamic acid"

Revision as of 13:13, 3 March 2021

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Salicylhydroxamic acid

Description

Salicylhydroxamic acid (SHAM; synonym: 2-Hydroxybenzohydroxamic acid N,2-Dihydroxybenzamide) is an inhibitor of the alternative oxidase (AOX). When AOX is blocked by SHAM, electrons are forced through the CIII-cytochrome c oxidase pathway, allowing observation of the operation of the CIII-CIV pathway without AOX activity.

Abbreviation: SHAM

Reference: Parrino_2000_J_Exp_Biol

Application in HRR

SHAM: Salicylhydroxamic acid (99%; HOC₆H₄CONHOH); Sigma S 607, 5 g, store at RT; M = 153.14 g·mol^-1.

SHAM is reported to be easily soluble in EtOH. So EtOH could be considered as a solvent, too. However, in Oroboros-lab SHAM in EtOH has not been tested yet.

Caution: Irritant!

Preparation of 250 mM stock solution (dissolved in DMSO):

Weigh 76.57 mg of SHAM.
Dissolve in 1950 µL of DMSO.
Divide into 0.2 mL portions
Store at -20 °C.

» O2k Manual Titrations: MiPNet09.12 O2k-Titrations

Titration volume: 8 µL
Final conc. in 2 mL O2k chamber: 1 mM

MitoPedia topics: Inhibitor

@@ Line 9: / Line 9: @@
 ::: '''SHAM: Salicylhydroxamic acid''' (99%; HOC<sub>6</sub>H<sub>4</sub>CONHOH); Sigma S 607, 5 g, store at RT; M = 153.14 g·mol<sup>-1</sup>.
-::: SHAM is reported to be easily soluble in EtOH. So EtOH could be considered as a solvent, too. However, in Oroboros-lab SHAM in EtOH has not been tested yet.
+:::: SHAM is reported to be easily soluble in EtOH. So EtOH could be considered as a solvent, too. However, in Oroboros-lab SHAM in EtOH has not been tested yet.
 ::::<span style="color:#8B008B"> '''Caution:''' Irritant!</span>