Savagner 2001 J Clin Endocrinol Metabol: Difference between revisions
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{{Publication | {{Publication | ||
|title=Savagner F, Franc B, Guyetant S, Rodien P, Reynier P, Malthiery Y (2001) Defective mitochondrial ATP synthesis in oxyphilic thyroid tumors. J | |title=Savagner F, Franc B, Guyetant S, Rodien P, Reynier P, Malthiery Y (2001) Defective mitochondrial ATP synthesis in oxyphilic thyroid tumors. J Clin Endocrinol Metabol 86:4920-25. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/11600563 PMDI: 11600563] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/11600563 PMDI: 11600563] | ||
|authors=Savagner F, Franc B, Guyetant S, Rodien P, Reynier P, Malthiery Y | |authors=Savagner F, Franc B, Guyetant S, Rodien P, Reynier P, Malthiery Y | ||
|year=2001 | |year=2001 | ||
|journal=J | |journal=J Clin Endocrinol Metabol | ||
|abstract=Oxyphilic tumors (oncocytomas or HΓΌrthle cell tumors) form a rare subgroup of thyroid tumors characterized by cells containing abundant mitochondria. The relationship between the mitochondrial proliferation and the pathogenesis of these tumors is unknown. We have assessed the expression of the mitochondrial'' ND2'' and ''ND5'' (subunits of the nicotinamide adenine dinucleotide dehydrogenase complex) genes and the nuclear UCP2 (uncoupling protein 2) gene in 22 oxyphilic thyroid tumors and matched controls. The consumption of oxygen in mitochondria from tumors was determined by polarography. ATP assays were used to explore the mitochondrial respiratory chain activity and the oxidative phosphorylation coupling in seven fresh thyroid tumors and controls. Adenosine triphosphate synthesis was significantly lower in all the tumors, compared with controls, suggesting that a coupling defect in oxidative phosphorylation may be a cause of mitochondrial hyperplasia in oxyphilic thyroid tumors. | |abstract=Oxyphilic tumors (oncocytomas or HΓΌrthle cell tumors) form a rare subgroup of thyroid tumors characterized by cells containing abundant mitochondria. The relationship between the mitochondrial proliferation and the pathogenesis of these tumors is unknown. We have assessed the expression of the mitochondrial'' ND2'' and ''ND5'' (subunits of the nicotinamide adenine dinucleotide dehydrogenase complex) genes and the nuclear UCP2 (uncoupling protein 2) gene in 22 oxyphilic thyroid tumors and matched controls. The consumption of oxygen in mitochondria from tumors was determined by polarography. ATP assays were used to explore the mitochondrial respiratory chain activity and the oxidative phosphorylation coupling in seven fresh thyroid tumors and controls. Adenosine triphosphate synthesis was significantly lower in all the tumors, compared with controls, suggesting that a coupling defect in oxidative phosphorylation may be a cause of mitochondrial hyperplasia in oxyphilic thyroid tumors. | ||
|mipnetlab= | |mipnetlab=FR Angers Andriantsitohaina R | ||
|discipline=Biomedicine | |discipline=Biomedicine | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration, mt-Biogenesis;mt-density, mt-Medicine | |||
|organism=Human | |||
|preparations=Isolated mitochondria | |||
|enzymes=TCA cycle and matrix dehydrogenases, Uncoupling protein | |||
|diseases=Cancer | |||
|topics=Coupling efficiency;uncoupling | |||
|couplingstates=OXPHOS | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|discipline=Biomedicine | |discipline=Biomedicine | ||
}} | }} |
Latest revision as of 15:47, 15 February 2018
Savagner F, Franc B, Guyetant S, Rodien P, Reynier P, Malthiery Y (2001) Defective mitochondrial ATP synthesis in oxyphilic thyroid tumors. J Clin Endocrinol Metabol 86:4920-25. |
Savagner F, Franc B, Guyetant S, Rodien P, Reynier P, Malthiery Y (2001) J Clin Endocrinol Metabol
Abstract: Oxyphilic tumors (oncocytomas or HΓΌrthle cell tumors) form a rare subgroup of thyroid tumors characterized by cells containing abundant mitochondria. The relationship between the mitochondrial proliferation and the pathogenesis of these tumors is unknown. We have assessed the expression of the mitochondrial ND2 and ND5 (subunits of the nicotinamide adenine dinucleotide dehydrogenase complex) genes and the nuclear UCP2 (uncoupling protein 2) gene in 22 oxyphilic thyroid tumors and matched controls. The consumption of oxygen in mitochondria from tumors was determined by polarography. ATP assays were used to explore the mitochondrial respiratory chain activity and the oxidative phosphorylation coupling in seven fresh thyroid tumors and controls. Adenosine triphosphate synthesis was significantly lower in all the tumors, compared with controls, suggesting that a coupling defect in oxidative phosphorylation may be a cause of mitochondrial hyperplasia in oxyphilic thyroid tumors.
β’ O2k-Network Lab: FR Angers Andriantsitohaina R
Labels: MiParea: Respiration, mt-Biogenesis;mt-density, mt-Medicine
Pathology: Cancer
Organism: Human
Preparation: Isolated mitochondria Enzyme: TCA cycle and matrix dehydrogenases, Uncoupling protein Regulation: Coupling efficiency;uncoupling Coupling state: OXPHOS
HRR: Oxygraph-2k