Difference between revisions of "Schneider 1949 J Biol Chem"
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# The mitochondrial fractions of these tissues had the highest oxalacetic oxidase activity of the fractions obtained, but this activity accounted for only about 45 and 30 per cent of the activity of the liver and kidney homogenates respectively. The activity of the other fractions was much lower that of the mitochondria, and the recovery of oxalacetic oxidase activity in the separate fractions was incomplete but approximately complete recovery was obtained when the enzymatic activities were determined on the recombined fractions. | # The mitochondrial fractions of these tissues had the highest oxalacetic oxidase activity of the fractions obtained, but this activity accounted for only about 45 and 30 per cent of the activity of the liver and kidney homogenates respectively. The activity of the other fractions was much lower that of the mitochondria, and the recovery of oxalacetic oxidase activity in the separate fractions was incomplete but approximately complete recovery was obtained when the enzymatic activities were determined on the recombined fractions. | ||
# The significance ofthese findings was discussed. It was concluded that the major portion of the enzymes of the oxalacetic oxidase system is probably associated with the mitochondria rat liver and kidney, but that the full activity of these enzymes is limited by the presence in the other fractions of accessory enzymes or coenzymes. | # The significance ofthese findings was discussed. It was concluded that the major portion of the enzymes of the oxalacetic oxidase system is probably associated with the mitochondria rat liver and kidney, but that the full activity of these enzymes is limited by the presence in the other fractions of accessory enzymes or coenzymes. | ||
|keywords=enzyme distribution, oxalacetic oxidase, | |keywords=enzyme distribution, oxalacetic oxidase, nucleic acids | ||
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{{Labeling | {{Labeling | ||
|organism=Rat | |||
|tissues=Hepatocyte; Liver, Kidney | |||
|preparations=Homogenate | |||
|additional=Made history | |additional=Made history | ||
}} | }} | ||
Revision as of 18:16, 8 June 2012
| Schneider WC, Potter VR (1949) Intracellular distribution of enzymes; the distribution of oxalacetic oxidase activity in rat liver and rat kidney fractions. J Biol Chem 177: 893-903. |
Schneider WC, Potter VR (1949) J Biol Chem
Abstract:
- Rat liver and kidney homogenates were fractionated by differential centrifugation into nuclear, mitochondrial, submicroscopic particle, and "soluble" protein fractions and the distribution of nucleic acids and oxalacetic oxidase in these fractions determined.
- The distribution of the nucleic acids in these fractions was to that described in previous work. Desoxypentose nucleic acid was recovered entirely in the nuclear fractions, while pentose nucleic acid (PNA) was found in all the fractions.
- The mitochondrial fractions of these tissues had the highest oxalacetic oxidase activity of the fractions obtained, but this activity accounted for only about 45 and 30 per cent of the activity of the liver and kidney homogenates respectively. The activity of the other fractions was much lower that of the mitochondria, and the recovery of oxalacetic oxidase activity in the separate fractions was incomplete but approximately complete recovery was obtained when the enzymatic activities were determined on the recombined fractions.
- The significance ofthese findings was discussed. It was concluded that the major portion of the enzymes of the oxalacetic oxidase system is probably associated with the mitochondria rat liver and kidney, but that the full activity of these enzymes is limited by the presence in the other fractions of accessory enzymes or coenzymes.
• Keywords: enzyme distribution, oxalacetic oxidase, nucleic acids
Labels:
Organism: Rat
Tissue;cell: Hepatocyte; Liver"Hepatocyte; Liver" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property., Kidney
Preparation: Homogenate
Made history
