Select plots - DatLab: Difference between revisions
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ย Contributed by [[Gnaiger Erich]] 2016-07-24 | ย Contributed by [[Gnaiger Erich]] 2016-07-24 | ||
__TOC__ | __TOC__ | ||
[[File:Select plots_Graph layout.png|900px|Mark statistics in DatLab 7]] | |||
== Action == | == Action == | ||
===Tab: Oxygen, O2=== | |||
:::# Go to the menue '''[Graph]/[Select plots]'''. The window Graph layout with tab 'Plots' is opened. ย | :::# Go to the menue '''[Graph]/[Select plots]'''. The window Graph layout with tab 'Plots' is opened. ย | ||
:::# '''Select''' the Graph for which the plots should be selected or edited. ย | :::# '''Select''' the Graph for which the plots should be selected or edited. ย | ||
:::# '''Tabs''': Select the channel for which plots should be selected. | :::# '''Tabs''': Select the channel for which plots should be selected.(Oxygen O2, Amperometric Amp, Potentiometric pX, System channels) | ||
:::# '''Tick boxes''' to select a plot for the Y1 or Y2 axis: The top two sections are for O2k chamber A, the two section on the bottom for O2k chamber B, in each case the first row is for the signal, the second for Flux/Slope. | :::# '''Tick boxes''' to select a plot for the Y1 (left) or Y2 (right) axis: The top two sections are for O2k chamber A, the two section on the bottom for O2k chamber B, in each case the first row is for the signal, the second for Flux/Slope. | ||
:::# '''Use default label''' | :::# '''Use default label''' to select the default axis label | ||
:::# '''Use custom label''' To define a different axis label, select and enter or edit a custom label. | |||
:::# '''Channel label'''Edit (for Amp and pX channels). | |||
:::# '''Color''' defines the color of the plot. | |||
:::# '''Width''' defines the line width of the plot. | |||
:::# '''Line style''': Select colour, thickness, and type (solid, dashed, etc). | :::# '''Line style''': Select colour, thickness, and type (solid, dashed, etc). | ||
:::# '''Unit''': A signal may be calibrated in various units ([ยตM; nM; mM), and the correct unit is selected here. This does not apply for oxygen, which is alway calibrated in units [ยตM]. | :::# '''Unit''': A signal may be calibrated in various units ([ยตM; nM; mM), and the correct unit is selected here. This does not apply for oxygen, which is alway calibrated in units [ยตM]. | ||
:::# '''Raw signal''': Selection for display of the raw signal changes automatically both, the signal and the slope. | :::# '''Raw signal''': Selection for display of the non-calibrated raw signal changes automatically both, the signal and the slope. | ||
:::# '''Flux per volume''' or '''Flux per mg''' ('''Flow per cells'''): Select the type of normalization of the Flux/Slope. | :::# '''Flux per volume''' or '''Flux per mg''' ('''Flow per cells'''): Select the type of normalization of the Flux/Slope. | ||
:::# '''Background corr.''': If backbround correction is selected, the background parameters are applied to display flux or flow. ยป''Further details:'' [[Flux / Slope]]. | :::# '''Background corr.''': If backbround correction is selected, the background parameters are applied to display flux or flow. ยป''Further details:'' [[Flux / Slope]]. | ||
:::# '''Layout type''' and '''Layout name''' are selected to '''Load''' or '''Save''' a Graph layout. | :::# '''Layout type''' and '''Layout name''' are selected to '''Load''' or '''Save''' a Graph layout. | ||
===Tab: System channels=== | |||
in progress | |||
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== Quantities, symbols and units == | == Quantities, symbols and units == |
Revision as of 14:54, 8 January 2018
Description
In the pull-down menue [Graph], Select plots opens the Graph layout window 'Plots'. For each graph, the plots shown with the Y1 or Y2 axis can be selected, axis labels and line styles can be defined, the unit for the calibrated signal can be changed, Flux/Slope can be chosen to be displayed as Flux per volume or as normalized specific flux/flow, the background correction can be switched on or off, and the channel can be selectively displayed as the raw signal. Graph layouts can be selected and loaded or a Graph layout may be saved. ยปCompare: Scaling - DatLab.
Abbreviation: Ctrl+F6
Reference: MiPNet19.18C DatLab-guide
MitoPedia O2k and high-resolution respirometry:
DatLab
Contributed by Gnaiger Erich 2016-07-24
Action
Tab: Oxygen, O2
- Go to the menue [Graph]/[Select plots]. The window Graph layout with tab 'Plots' is opened.
- Select the Graph for which the plots should be selected or edited.
- Tabs: Select the channel for which plots should be selected.(Oxygen O2, Amperometric Amp, Potentiometric pX, System channels)
- Tick boxes to select a plot for the Y1 (left) or Y2 (right) axis: The top two sections are for O2k chamber A, the two section on the bottom for O2k chamber B, in each case the first row is for the signal, the second for Flux/Slope.
- Use default label to select the default axis label
- Use custom label To define a different axis label, select and enter or edit a custom label.
- Channel labelEdit (for Amp and pX channels).
- Color defines the color of the plot.
- Width defines the line width of the plot.
- Line style: Select colour, thickness, and type (solid, dashed, etc).
- Unit: A signal may be calibrated in various units ([ยตM; nM; mM), and the correct unit is selected here. This does not apply for oxygen, which is alway calibrated in units [ยตM].
- Raw signal: Selection for display of the non-calibrated raw signal changes automatically both, the signal and the slope.
- Flux per volume or Flux per mg (Flow per cells): Select the type of normalization of the Flux/Slope.
- Background corr.: If backbround correction is selected, the background parameters are applied to display flux or flow. ยปFurther details: Flux / Slope.
- Layout type and Layout name are selected to Load or Save a Graph layout.
Tab: System channels
in progress
Quantities, symbols and units
Quantity | Symbol | Units | Accurate results depend on |
---|---|---|---|
O2 raw signal | RO2 | V | |
O2 concentrationa | cO2 | ยตM | a) Oxygen calibration; edit in [F5], including the oxygen solubility factor of the medium, FM. |
O2 pressureb | pO2 | kPa (mmHg) | b) Oxygen calibration; edit in [F5], independent of the oxygen solubility factor of the medium, FM. |
O2 slope negativec | O2 slope negative | pmolโs-1โml-1 | c) In addition, on correction for instrumental O2 background flux; edit in [Flux / Slope]. |
O2 flux per volumec | JV,O2 | pmolโs-1โml-1 | |
O2 flow per cellsd | IO2 | pmolโs-1โ10-6 | d) In addition, on information about sample concentration; edit [F3]: Million cells. |
O2 flux per massd | JO2 | pmolโs-1โmg-1 | d) In addition, on information about sample concentration; edit [F3]: mg. |
O2 flow per unitd | IO2 | pmolโs-1โml-1โU-1 | d) In addition, on information about sample concentration; edit [F3]: Unit. |
Flux control ratio (per reference state)e | FCR | e) Independent on sample concentration; edit [F3]: Reference state. |
- Note: Oxygen flux is expressed in moles of oxygen, O2, independent of choosing a plot of oxygen pressure or concentration.