Difference between revisions of "Sodium phosphate buffer"
From Bioblast
| Line 2: | Line 2: | ||
|abbr=Na-PB | |abbr=Na-PB | ||
|description=Sodium phosphate buffer, '''Na-PB''', for [[HRR]] with freeze-dried baker´s yeast. | |description=Sodium phosphate buffer, '''Na-PB''', for [[HRR]] with freeze-dried baker´s yeast. | ||
}} | }} | ||
{{MitoPedia O2k and high-resolution respirometry|type=Chemicals | {{MitoPedia O2k and high-resolution respirometry|type=Chemicals | ||
| Line 12: | Line 7: | ||
{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic=Medium | |mitopedia topic=Medium | ||
}} | }} | ||
== Application in [[ | == Application in [[HRR]] == | ||
* ''' | * '''Oxygen solubility factor''' of Na-PB = '''0.95''' | ||
== Na-PB (50 mM, pH 7.1)== | == Na-PB (50 mM, pH 7.1)== | ||
Dissolve 3.9 g NaH<sub>2</sub>PO<sub>4</sub> x 2 H<sub>2</sub>O (Sigma 71505, FW = 156.01 ) in 500 | Dissolve 3.9 g NaH<sub>2</sub>PO<sub>4</sub> x 2 H<sub>2</sub>O (Sigma 71505, FW = 156.01 ) in 500 mL a.dest and adjust pH with 10 M NaOH (Sigma 72068, FW = 40.00) to 7.1. | ||
Revision as of 13:32, 17 March 2019
- high-resolution terminology - matching measurements at high-resolution
Sodium phosphate buffer
Description
Sodium phosphate buffer, Na-PB, for HRR with freeze-dried baker´s yeast.
Abbreviation: Na-PB
MitoPedia topics: Media for respirometry
Application in HRR
- Oxygen solubility factor of Na-PB = 0.95
Na-PB (50 mM, pH 7.1)
Dissolve 3.9 g NaH2PO4 x 2 H2O (Sigma 71505, FW = 156.01 ) in 500 mL a.dest and adjust pH with 10 M NaOH (Sigma 72068, FW = 40.00) to 7.1.
