Difference between revisions of "Sodium phosphate buffer"
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== Na-PB (50 mM, pH 7.1)== | == Na-PB (50 mM, pH 7.1)== | ||
Dissolve 3.9 g NaH<sub>2</sub>PO<sub>4</sub> x 2 H<sub>2</sub>O in 500 ml | Dissolve 3.9 g NaH<sub>2</sub>PO<sub>4</sub> x 2 H<sub>2</sub>O (Sigma 71505, FW = 156.01 ) in 500 ml A. dest and adjust pH with 10 M NaOH (Sigma 72068, FW = 40.00)to 7.1. | ||
Revision as of 13:56, 23 April 2012
- high-resolution terminology - matching measurements at high-resolution
Sodium phosphate buffer
Description
Sodium phosphate buffer, Na-PB, for HRR with freeze-dried baker´s yeast
Abbreviation: Na-PB
MitoPedia methods:
Respirometry
MitoPedia topics:
Media for respirometry
Application in HRR
- experimental temperature = 28°C
- Oxygen solubility factor of Na-PB = 0.95
Na-PB (50 mM, pH 7.1)
Dissolve 3.9 g NaH2PO4 x 2 H2O (Sigma 71505, FW = 156.01 ) in 500 ml A. dest and adjust pH with 10 M NaOH (Sigma 72068, FW = 40.00)to 7.1.
