Difference between revisions of "Sondheimer 2010 Biochemistry"
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{{Publication | {{Publication | ||
|title=Sondheimer N, Fang JK, Polyak E, Falk MJ, Avadhani NG (2010) Leucine-rich pentatricopeptide-repeat containing protein regulates mitochondrial transcription. Biochemistry 49 | |title=Sondheimer N, Fang JK, Polyak E, Falk MJ, Avadhani NG (2010) Leucine-rich pentatricopeptide-repeat containing protein regulates mitochondrial transcription. Biochemistry 49:7467-73. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed | |info=[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2932791/?tool=pubmed PMID: 20677761 Open Access] | ||
|authors=Sondheimer N, Fang JK, Polyak E, Falk MJ, Avadhani NG | |authors=Sondheimer N, Fang JK, Polyak E, Falk MJ, Avadhani NG | ||
|year=2010 | |year=2010 | ||
|journal=Biochemistry | |journal=Biochemistry | ||
|abstract=Mitochondrial function depends upon the coordinated expression of the mitochondrial and nuclear genomes. Although the basal factors that carry out the process of mitochondrial transcription are known, the regulation of this process is incompletely understood. To further our understanding of mitochondrial gene regulation, we identified proteins that bound to the previously described point of termination for the major mRNA-coding transcript H2. One was the leucine-rich pentatricopeptide-repeat containing protein (LRPPRC), which has been linked to the French-Canadian variant of Leigh syndrome. Cells with reduced expression of LRPPRC had a reduction in oxygen consumption. The expression of mitochondrial mRNA and tRNA was dependent upon LRPPRC levels, but reductions in LRPPRC did not affect the expression of mitochondrial rRNA. Reduction of LRPPRC levels interfered with mitochondrial transcription in vitro but did not affect the stability of mitochondrial mRNAs or alter the expression of nuclear genes responsible for mitochondrial transcription in vivo. These findings demonstrate the control of mitochondrial mRNA synthesis by a protein that has an established role in regulating nuclear transcription and a link to mitochondrial disease. | |abstract=Mitochondrial function depends upon the coordinated expression of the mitochondrial and nuclear genomes. Although the basal factors that carry out the process of mitochondrial transcription are known, the regulation of this process is incompletely understood. To further our understanding of mitochondrial gene regulation, we identified proteins that bound to the previously described point of termination for the major mRNA-coding transcript H2. One was the leucine-rich pentatricopeptide-repeat containing protein (LRPPRC), which has been linked to the French-Canadian variant of Leigh syndrome. Cells with reduced expression of LRPPRC had a reduction in oxygen consumption. The expression of mitochondrial mRNA and tRNA was dependent upon LRPPRC levels, but reductions in LRPPRC did not affect the expression of mitochondrial rRNA. Reduction of LRPPRC levels interfered with mitochondrial transcription ''in vitro'' but did not affect the stability of mitochondrial mRNAs or alter the expression of nuclear genes responsible for mitochondrial transcription ''in vivo''. These findings demonstrate the control of mitochondrial mRNA synthesis by a protein that has an established role in regulating nuclear transcription and a link to mitochondrial disease. | ||
|keywords=HeLa cells | |keywords=HeLa cells | ||
|mipnetlab=US PA Philadelphia Falk MJ | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|organism=Human | |||
|preparations=Intact cells | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
}} | }} | ||
Latest revision as of 12:56, 19 March 2015
| Sondheimer N, Fang JK, Polyak E, Falk MJ, Avadhani NG (2010) Leucine-rich pentatricopeptide-repeat containing protein regulates mitochondrial transcription. Biochemistry 49:7467-73. |
Sondheimer N, Fang JK, Polyak E, Falk MJ, Avadhani NG (2010) Biochemistry
Abstract: Mitochondrial function depends upon the coordinated expression of the mitochondrial and nuclear genomes. Although the basal factors that carry out the process of mitochondrial transcription are known, the regulation of this process is incompletely understood. To further our understanding of mitochondrial gene regulation, we identified proteins that bound to the previously described point of termination for the major mRNA-coding transcript H2. One was the leucine-rich pentatricopeptide-repeat containing protein (LRPPRC), which has been linked to the French-Canadian variant of Leigh syndrome. Cells with reduced expression of LRPPRC had a reduction in oxygen consumption. The expression of mitochondrial mRNA and tRNA was dependent upon LRPPRC levels, but reductions in LRPPRC did not affect the expression of mitochondrial rRNA. Reduction of LRPPRC levels interfered with mitochondrial transcription in vitro but did not affect the stability of mitochondrial mRNAs or alter the expression of nuclear genes responsible for mitochondrial transcription in vivo. These findings demonstrate the control of mitochondrial mRNA synthesis by a protein that has an established role in regulating nuclear transcription and a link to mitochondrial disease. • Keywords: HeLa cells
• O2k-Network Lab: US PA Philadelphia Falk MJ
Labels:
Organism: Human
Preparation: Intact cells
HRR: Oxygraph-2k
