Stampley 2023 Physiol Rep: Difference between revisions
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|title=Stampley JE, Cho E, Wang H, Theall B, Johannsen NM, Spielmann G, Irving BA (2023) Impact of maximal exercise on immune cell mobilization and bioenergetics. https://doi.org/10.14814/phy2.15753 | |title=Stampley JE, Cho E, Wang H, Theall B, Johannsen NM, Spielmann G, Irving BA (2023) Impact of maximal exercise on immune cell mobilization and bioenergetics. https://doi.org/10.14814/phy2.15753 | ||
|info=Physiol Rep 11:e15753. [https://pubmed.ncbi.nlm.nih.gov/37312242 PMID: 37312242 Open Access] | |info=Physiol Rep 11:e15753. [https://pubmed.ncbi.nlm.nih.gov/37312242 PMID: 37312242 Open Access] | ||
|authors=Stampley | |authors=Stampley James E, Cho Eunhan, Wang Haoyan, Theall Bailey, Johannsen Neil M, Spielmann Guillaume, Irving Brian A | ||
|year=2023 | |year=2023 | ||
|journal=Physiol Rep | |journal=Physiol Rep | ||
|abstract=Acute aerobic exercise increases the number and proportions of circulating peripheral blood mononuclear cells (PMBC) and can alter PBMC mitochondrial bioenergetics. In this study, we aimed to examine the impact of a maximal exercise bout on immune cell metabolism in collegiate swimmers. Eleven (7 M/4F) collegiate swimmers completed a maximal exercise test to measure anaerobic power and capacity. Pre- and postexercise PBMCs were isolated to measure the immune cell phenotypes and mitochondrial bioenergetics using flow cytometry and high-resolution respirometry. The maximal exercise bout increased circulating levels of PBMCs, particularly in central memory (KLRG1+/CD57-) and senescent (KLRG1+/CD57+) CD8+ T cells, whether measured as a % of PMBCs or as absolute concentrations (all p < 0.05). At the cellularlevel, the routine oxygen flow ( | |abstract=Acute aerobic exercise increases the number and proportions of circulating peripheral blood mononuclear cells (PMBC) and can alter PBMC mitochondrial bioenergetics. In this study, we aimed to examine the impact of a maximal exercise bout on immune cell metabolism in collegiate swimmers. Eleven (7 M/4F) collegiate swimmers completed a maximal exercise test to measure anaerobic power and capacity. Pre- and postexercise PBMCs were isolated to measure the immune cell phenotypes and mitochondrial bioenergetics using flow cytometry and high-resolution respirometry. The maximal exercise bout increased circulating levels of PBMCs, particularly in central memory (KLRG1+/CD57-) and senescent (KLRG1+/CD57+) CD8+ T cells, whether measured as a % of PMBCs or as absolute concentrations (all p < 0.05). At the cellularlevel, the routine oxygen flow (IO<sub>2</sub> [pmolยทs<sup>-1</sup> ยท10<sup>6</sup> PBMCs<sup>-1</sup> ]) increased following maximal exercise (p = 0.042); however, there were no effects of exercise on the IO<sub>2</sub> measured under the LEAK, oxidative phosphorylation (OXPHOS), or electron transfer (ET) capacities. There were exercise-induced increases in the tissue-level oxygen flow (IO<sub>2</sub>-tissue [pmolยทs<sup>-1</sup> ยทmL blood<sup>-1</sup> ]) for all respiratory states (all p < 0.01), except for the LEAK state, after accounting for the mobilization of PBMCs. Future subtype-specific studies are needed to characterize further maximal exercise's true impact on immune cell bioenergetics. | ||
|keywords=PBMC, Athletes, Inflammation, Mitochondria, Oxygraph, Respirometry | |||
|editor=[[Plangger M]] | |editor=[[Plangger M]] | ||
|mipnetlab=US LA Baton Rouge Irving BA | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration | |area=Respiration, Exercise physiology;nutrition;life style | ||
|organism=Human | |||
|tissues=Blood cells | |||
|preparations=Permeabilized cells, Intact cells | |||
|couplingstates=LEAK, ROUTINE, OXPHOS, ET | |||
|pathways=N, NS, ROX | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=2023-06 | |additional=2023-06, PBMCs | ||
}} | }} |
Latest revision as of 15:40, 20 June 2023
Stampley JE, Cho E, Wang H, Theall B, Johannsen NM, Spielmann G, Irving BA (2023) Impact of maximal exercise on immune cell mobilization and bioenergetics. https://doi.org/10.14814/phy2.15753 |
ยป Physiol Rep 11:e15753. PMID: 37312242 Open Access
Stampley James E, Cho Eunhan, Wang Haoyan, Theall Bailey, Johannsen Neil M, Spielmann Guillaume, Irving Brian A (2023) Physiol Rep
Abstract: Acute aerobic exercise increases the number and proportions of circulating peripheral blood mononuclear cells (PMBC) and can alter PBMC mitochondrial bioenergetics. In this study, we aimed to examine the impact of a maximal exercise bout on immune cell metabolism in collegiate swimmers. Eleven (7 M/4F) collegiate swimmers completed a maximal exercise test to measure anaerobic power and capacity. Pre- and postexercise PBMCs were isolated to measure the immune cell phenotypes and mitochondrial bioenergetics using flow cytometry and high-resolution respirometry. The maximal exercise bout increased circulating levels of PBMCs, particularly in central memory (KLRG1+/CD57-) and senescent (KLRG1+/CD57+) CD8+ T cells, whether measured as a % of PMBCs or as absolute concentrations (all p < 0.05). At the cellularlevel, the routine oxygen flow (IO2 [pmolยทs-1 ยท106 PBMCs-1 ]) increased following maximal exercise (p = 0.042); however, there were no effects of exercise on the IO2 measured under the LEAK, oxidative phosphorylation (OXPHOS), or electron transfer (ET) capacities. There were exercise-induced increases in the tissue-level oxygen flow (IO2-tissue [pmolยทs-1 ยทmL blood-1 ]) for all respiratory states (all p < 0.01), except for the LEAK state, after accounting for the mobilization of PBMCs. Future subtype-specific studies are needed to characterize further maximal exercise's true impact on immune cell bioenergetics. โข Keywords: PBMC, Athletes, Inflammation, Mitochondria, Oxygraph, Respirometry โข Bioblast editor: Plangger M โข O2k-Network Lab: US LA Baton Rouge Irving BA
Labels: MiParea: Respiration, Exercise physiology;nutrition;life style
Organism: Human
Tissue;cell: Blood cells
Preparation: Permeabilized cells, Intact cells
Coupling state: LEAK, ROUTINE, OXPHOS, ET
Pathway: N, NS, ROX
HRR: Oxygraph-2k
2023-06, PBMCs