Difference between revisions of "Substrate-uncoupler-inhibitor titration"
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|description=Mitochondrial '''Substrate-uncoupler-inhibitor titration''' ('''SUIT''') [[MitoPedia: SUIT |protocols]] are used with [[mt-preparations]] to study respiratory control in a sequence of coupling and substrates states induced by multiple titrations within a single experimental [[assay]]. | |description=Mitochondrial '''Substrate-uncoupler-inhibitor titration''' ('''SUIT''') [[MitoPedia: SUIT |protocols]] are used with [[mt-preparations]] to study respiratory control in a sequence of coupling and substrates states induced by multiple titrations within a single experimental [[assay]]. | ||
|info=[[Gnaiger 2014 MitoPathways]], [[MiPNet09.12]], [[MitoPedia: SUIT]] | |info=[[Gnaiger 2014 MitoPathways]], [[MiPNet09.12]], [[MitoPedia: SUIT]] | ||
}} | }} | ||
{{MitoPedia concepts | {{MitoPedia concepts | ||
|mitopedia concept=MiP concept, SUIT concept | |mitopedia concept=MiP concept, SUIT concept | ||
}} | }} | ||
{{MitoPedia methods | {{MitoPedia methods | ||
|mitopedia method=Respirometry | |mitopedia method=Respirometry | ||
}} | }} | ||
{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic=Inhibitor, Substrate and metabolite, Uncoupler | |mitopedia topic=Inhibitor, Substrate and metabolite, Uncoupler | ||
}} | }} | ||
Contributed by [[Gnaiger E]] 2010-09-18, edited 2016-01-24. | Contributed by [[Gnaiger E]] 2010-09-18, edited 2016-01-24. | ||
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:::: SUIT protocols have been developed for the first time on the basis of [[high-resolution respirometry]], since their application depends critically on long-term stability of the [[polarographic oxygen sensor]] (experiments may be extended over 1 to 2 hours), highly diluted sample to avoid fast oxygen depletion in the closed chamber, and the possibility for re-oxygenations to extend measurement of oxygen flux. The mitochondrial preparation must be stable over such experimental periods of time, hence specific mitochondrial respiration media (MiR05: [[Gnaiger_2000_MitoInTheCold]]; [[MiR06]]) have been developed to maintain respiratory capacity during application of mitochondrial SUIT protocols at physiological temperature. | :::: SUIT protocols have been developed for the first time on the basis of [[high-resolution respirometry]], since their application depends critically on long-term stability of the [[polarographic oxygen sensor]] (experiments may be extended over 1 to 2 hours), highly diluted sample to avoid fast oxygen depletion in the closed chamber, and the possibility for re-oxygenations to extend measurement of oxygen flux. The mitochondrial preparation must be stable over such experimental periods of time, hence specific mitochondrial respiration media (MiR05: [[Gnaiger_2000_MitoInTheCold]]; [[MiR06]]) have been developed to maintain respiratory capacity during application of mitochondrial SUIT protocols at physiological temperature. | ||
::: Attempts are in progress to develop a database of SUIT protocols | |||
::::* [[SUIT protocol library]] | |||
::::* [[MitoPedia:_SUIT#SUIT_protocols |MitoPedia: SUIT protocols]] | |||
Revision as of 08:52, 15 May 2016
- high-resolution terminology - matching measurements at high-resolution
Substrate-uncoupler-inhibitor titration
Description
Mitochondrial Substrate-uncoupler-inhibitor titration (SUIT) protocols are used with mt-preparations to study respiratory control in a sequence of coupling and substrates states induced by multiple titrations within a single experimental assay.
Abbreviation: SUIT
Reference: Gnaiger 2014 MitoPathways, MiPNet09.12, MitoPedia: SUIT
MitoPedia concepts:
MiP concept,
SUIT concept
MitoPedia methods:
Respirometry
MitoPedia topics:
Inhibitor,
Substrate and metabolite,
Uncoupler
Contributed by Gnaiger E 2010-09-18, edited 2016-01-24.
SUIT protocols
- SUIT protocols have been developed for the first time on the basis of high-resolution respirometry, since their application depends critically on long-term stability of the polarographic oxygen sensor (experiments may be extended over 1 to 2 hours), highly diluted sample to avoid fast oxygen depletion in the closed chamber, and the possibility for re-oxygenations to extend measurement of oxygen flux. The mitochondrial preparation must be stable over such experimental periods of time, hence specific mitochondrial respiration media (MiR05: Gnaiger_2000_MitoInTheCold; MiR06) have been developed to maintain respiratory capacity during application of mitochondrial SUIT protocols at physiological temperature.
- Attempts are in progress to develop a database of SUIT protocols
