Difference between revisions of "Substrate-uncoupler-inhibitor titration"
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{{MitoPedia | {{MitoPedia | ||
|abbr=SUIT | |abbr=SUIT | ||
|description='''SUIT''' protocols are used with mt-preparations to study respiratory control in a sequence of coupling and substrates states induced by multiple titrations. | |description=Mitochondrial '''SUIT''' protocols are used with mt-preparations to study respiratory control in a sequence of coupling and substrates states induced by multiple titrations. | ||
|info=[[MiPNet12.12]], [[MiPNet09.12]], [[Gnaiger_2007_MitoPathways]], [[Gnaiger_2009_IJBCB]] | |info=[[MiPNet12.12]], [[MiPNet09.12]], [[Gnaiger_2007_MitoPathways]], [[Gnaiger_2009_IJBCB]] | ||
|type=Respiration | |||
}} | |||
{{MitoPedia topics | |||
|mitopedia topic=Respiratory state | |||
|type=Respiration | |type=Respiration | ||
}} | }} | ||
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|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
|preparations=Isolated Mitochondria, Permeabilized Cell or Tissue; Homogenate | |preparations=Isolated Mitochondria, Permeabilized Cell or Tissue; Homogenate | ||
|topics=Respiration; OXPHOS; ETS Capacity, Flux Control; Additivity; Threshold; Excess Capacity | |||
|topics=Respiration; OXPHOS; ETS Capacity, Flux Control; Additivity; Threshold; Excess Capacity | |||
}} | }} | ||
SUIT protocols have been developed for the first time on the basis of [[high-resolution respirometry]], since their application depends critically on long-term stability of the [[polarographic oxygen sensor]] (experiments may be extended over 1 to 2 hours), highly diluted sample to avoid fast oxygen depletion in the closed chamber, and the possibility for re-oxygenations to extend measurement of oxygen flux. The mitochondrial preparation must be stable over such experimental periods of time, hence specific mitochondrial respiration media (MiR05: [[Gnaiger_2000_MitoInTheCold]]; [[MiR06]]) have been developed to maintain respiratory capacity during application of SUIT protocols at physiological temperature ([[Gnaiger_2007_MitoPathways]]). | SUIT protocols have been developed for the first time on the basis of [[high-resolution respirometry]], since their application depends critically on long-term stability of the [[polarographic oxygen sensor]] (experiments may be extended over 1 to 2 hours), highly diluted sample to avoid fast oxygen depletion in the closed chamber, and the possibility for re-oxygenations to extend measurement of oxygen flux. The mitochondrial preparation must be stable over such experimental periods of time, hence specific mitochondrial respiration media (MiR05: [[Gnaiger_2000_MitoInTheCold]]; [[MiR06]]) have been developed to maintain respiratory capacity during application of mitochondrial SUIT protocols at physiological temperature ([[Gnaiger_2007_MitoPathways]]). | ||
Revision as of 05:42, 10 June 2011
- high-resolution terminology - matching measurements at high-resolution
Substrate-uncoupler-inhibitor titration
Description
Mitochondrial SUIT protocols are used with mt-preparations to study respiratory control in a sequence of coupling and substrates states induced by multiple titrations.
Abbreviation: SUIT
Reference: MiPNet12.12, MiPNet09.12, Gnaiger_2007_MitoPathways, Gnaiger_2009_IJBCB
MitoPedia topics: "Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
Respiratory state"Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
Labels:
Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property., Permeabilized Cell or Tissue; Homogenate"Permeabilized Cell or Tissue; Homogenate" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Flux Control; Additivity; Threshold; Excess Capacity"Flux Control; Additivity; Threshold; Excess Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
SUIT protocols have been developed for the first time on the basis of high-resolution respirometry, since their application depends critically on long-term stability of the polarographic oxygen sensor (experiments may be extended over 1 to 2 hours), highly diluted sample to avoid fast oxygen depletion in the closed chamber, and the possibility for re-oxygenations to extend measurement of oxygen flux. The mitochondrial preparation must be stable over such experimental periods of time, hence specific mitochondrial respiration media (MiR05: Gnaiger_2000_MitoInTheCold; MiR06) have been developed to maintain respiratory capacity during application of mitochondrial SUIT protocols at physiological temperature (Gnaiger_2007_MitoPathways).
