Difference between revisions of "TPP+ inhibitory effect"
| Line 1: | Line 1: | ||
{{MitoPedia | {{MitoPedia | ||
|description=A major | |description=A major task in establishing a procedure for measurement of [[mitochondrial membrane potential]] using probe molecules is the evaluation of inhibitory concentrations of the probe molecule on the activity of respiration. The '''TPP<sup>+</sup> inhibitory effect''' (this also applies to TPMP<sup>+</sup> and other indicator molecules) is frequently ignored. Accurate knowledge of a threshold concentration is required to evaluate the necessary limit of detection of TPP<sup>+</sup>, and for restriction of experimental TPP<sup>+</sup> concentrations below the inhibitory range. | ||
|info=MiPNet14.05 | |info=[[MiPNet14.05]] | ||
}} | }} | ||
<br /> | |||
{{Template:Keywords Membrane potential}} | |||
{{Labeling | {{Labeling | ||
|instruments=MultiSensor pH; TPP; NO, Chemicals; Media | |instruments=MultiSensor pH; TPP; NO, Chemicals; Media | ||
|topics=Respiration; OXPHOS; ETS Capacity | |topics=Respiration; OXPHOS; ETS Capacity | ||
}} | }} | ||
Revision as of 09:26, 10 September 2018
- high-resolution terminology - matching measurements at high-resolution
TPP+ inhibitory effect
Description
A major task in establishing a procedure for measurement of mitochondrial membrane potential using probe molecules is the evaluation of inhibitory concentrations of the probe molecule on the activity of respiration. The TPP+ inhibitory effect (this also applies to TPMP+ and other indicator molecules) is frequently ignored. Accurate knowledge of a threshold concentration is required to evaluate the necessary limit of detection of TPP+, and for restriction of experimental TPP+ concentrations below the inhibitory range.
Reference: MiPNet14.05
Template:Keywords Membrane potential
Labels:
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: MultiSensor pH; TPP; NO"MultiSensor pH; TPP; NO" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
