Tahara 2009 Free Radic Biol Med: Difference between revisions
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|title=Tahara EB, Navarete FD, Kowaltowski AJ (2009) Tissue-, substrate-, and site-specific characteristics of mitochondrial reactive oxygen species generation. Free Radic Biol Med 46:1283-97. | |title=Tahara EB, Navarete FD, Kowaltowski AJ (2009) Tissue-, substrate-, and site-specific characteristics of mitochondrial reactive oxygen species generation. Free Radic Biol Med 46:1283-97. | ||
|info=[https://www.ncbi.nlm.nih.gov/pubmed/19245829 PMID: 19245829] | |info=[https://www.ncbi.nlm.nih.gov/pubmed/19245829 PMID: 19245829 Open Access] | ||
|authors=Tahara EB, Navarete FD, Kowaltowski AJ | |authors=Tahara EB, Navarete FD, Kowaltowski AJ | ||
|year=2009 | |year=2009 | ||
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|mipnetlab=BR Sao Paulo Kowaltowski AJ | |mipnetlab=BR Sao Paulo Kowaltowski AJ | ||
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== Cited by == | |||
{{Template:Cited by Komlodi 2021 MitoFit AmR}} | |||
{{Labeling | {{Labeling | ||
|area=Respiration | |area=Respiration |
Latest revision as of 12:42, 2 August 2023
Tahara EB, Navarete FD, Kowaltowski AJ (2009) Tissue-, substrate-, and site-specific characteristics of mitochondrial reactive oxygen species generation. Free Radic Biol Med 46:1283-97. |
Tahara EB, Navarete FD, Kowaltowski AJ (2009) Free Radic Biol Med
Abstract: Reactive oxygen species are a by-product of mitochondrial oxidative phosphorylation, derived from a small quantity of superoxide radicals generated during electron transport. We conducted a comprehensive and quantitative study of oxygen consumption, inner membrane potentials, and H2O2 release in mitochondria isolated from rat brain, heart, kidney, liver, and skeletal muscle, using various respiratory substrates (alpha-ketoglutarate, glutamate, succinate, glycerol phosphate, and palmitoyl carnitine). The locations and properties of reactive oxygen species formation were determined using oxidative phosphorylation and the respiratory chain modulators oligomycin, rotenone, myxothiazol, and antimycin A and the uncoupler CCCP. We found that in mitochondria isolated from most tissues incubated under physiologically relevant conditions, reactive oxygen release accounts for 0.1-0.2% of O2 consumed. Our findings support an important participation of flavoenzymes and complex III and a substantial role for reverse electron transport to complex I as reactive oxygen species sources. Our results also indicate that succinate is an important substrate for isolated mitochondrial reactive oxygen production in brain, heart, kidney, and skeletal muscle, whereas fatty acids generate significant quantities of oxidants in kidney and liver. Finally, we found that increasing respiratory rates is an effective way to prevent mitochondrial oxidant release under many, but not all, conditions. Altogether, our data uncover and quantify many tissue-, substrate-, and site-specific characteristics of mitochondrial ROS release. โข Keywords: Amplex Red โข Bioblast editor: Krumschnabel G โข O2k-Network Lab: BR Sao Paulo Kowaltowski AJ
Cited by
- Komlรณdi T, Schmitt S, Zdrazilova L, Donnelly C, Zischka H, Gnaiger E. Oxygen dependence of hydrogen peroxide production in isolated mitochondria and permeabilized cells. MitoFit Preprints (in prep).
Labels: MiParea: Respiration
Stress:Oxidative stress;RONS Organism: Rat Tissue;cell: Heart, Skeletal muscle, Nervous system, Liver, Kidney Preparation: Isolated mitochondria
Regulation: Substrate Coupling state: LEAK, OXPHOS, ET Pathway: F, N, S, Gp, NS, ROX HRR: Oxygraph-2k
MitoFit 2021 AmR