Difference between revisions of "Talk:Glucose"
From Bioblast
| Line 1: | Line 1: | ||
__TOC__ | __TOC__ | ||
== MiPNet discussion forum: | == MiPNet discussion forum: glucose oxidation in skeletal muscle (2013-10-29) == | ||
=== Dominik Pesta === | === Dominik Pesta === | ||
Has anyone ever looked at glucose oxidation in intact skeletal muscle fibres (of mouse muscle)? I have tried with a Krebs Henseleit Buffer (no substrates) and Malate and/or Pyruvate followed by glucose (basal) and insulin titration (insulin stimulated) but do not get any stimulation of respiration. Although malate will not permeate the cell, I would at least expect some stimulation with glucose. Any thoughts on that? | Has anyone ever looked at glucose oxidation in intact skeletal muscle fibres (of mouse muscle)? I have tried with a Krebs Henseleit Buffer (no substrates) and Malate and/or Pyruvate followed by glucose (basal) and insulin titration (insulin stimulated) but do not get any stimulation of respiration. Although malate will not permeate the cell, I would at least expect some stimulation with glucose. Any thoughts on that? | ||
Revision as of 12:34, 29 October 2013
MiPNet discussion forum: glucose oxidation in skeletal muscle (2013-10-29)
Dominik Pesta
Has anyone ever looked at glucose oxidation in intact skeletal muscle fibres (of mouse muscle)? I have tried with a Krebs Henseleit Buffer (no substrates) and Malate and/or Pyruvate followed by glucose (basal) and insulin titration (insulin stimulated) but do not get any stimulation of respiration. Although malate will not permeate the cell, I would at least expect some stimulation with glucose. Any thoughts on that?
