Talk:Measuring hydrogen peroxide: Difference between revisions
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== MiPNet discussion forum: hydrogen peroxide production in intact muscle fibers (2013-10-25) == | == MiPNet discussion forum: hydrogen peroxide production in intact muscle fibers (2013-10-25) == | ||
=== Marcus Olivera === | === Marcus Olivera === | ||
I´ve been working on intact insect flight muscle to evaluate respiratory capacities but I´m experiencing many problems in detecting changes in rates of Amplex red fluorescence on physically permeabilized flight muscle. Does anyone know a suitable method to detect hydrogen peroxide generation on intact muscle fibers (not isolated mitochondria)? | I´ve been working on intact insect flight muscle to evaluate respiratory capacities but I´m experiencing many problems in detecting changes in rates of Amplex red fluorescence on physically permeabilized flight muscle. Does anyone know a suitable method to detect hydrogen peroxide generation on intact muscle fibers (not isolated mitochondria)? | ||
=== Nicolas Pichaud === | |||
We are presently doing a test with a marker called CellRox from Molecular Probesbut you need a confocal microscope. It is working pretty well so far, we just need to do a bit more of optimization... I'll send you the protocol when it is ready. | |||
Revision as of 14:03, 25 October 2013
MiPNet discussion forum: hydrogen peroxide production in intact muscle fibers (2013-10-25)
Marcus Olivera
I´ve been working on intact insect flight muscle to evaluate respiratory capacities but I´m experiencing many problems in detecting changes in rates of Amplex red fluorescence on physically permeabilized flight muscle. Does anyone know a suitable method to detect hydrogen peroxide generation on intact muscle fibers (not isolated mitochondria)?
Nicolas Pichaud
We are presently doing a test with a marker called CellRox from Molecular Probesbut you need a confocal microscope. It is working pretty well so far, we just need to do a bit more of optimization... I'll send you the protocol when it is ready.
