Difference between revisions of "Talk:O2k-Chambers"
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::::# The oxygen concentration should decrease overtime due to the O<sub>2</sub> consumed by [[OroboPOS]] as it is shown in the figure below. In this case, the chamber is assembled properly. | ::::# The oxygen concentration should decrease overtime due to the O<sub>2</sub> consumed by [[OroboPOS]] as it is shown in the figure below. In this case, the chamber is assembled properly. | ||
::::# If the oxygen concentration increases over time, a reassemble of the chamber is needed. | ::::# If the oxygen concentration increases over time, a reassemble of the chamber is needed. | ||
::::[[User:CecattoC|CecattoC]] ([[User talk:CecattoC|talk]]) 15:52, 19 March 2020 (CET). So, it should be done every reassemble otherwise nobody will noticed that? How frequent you received cases with this or noticed leaky chamber in our lab? |
Revision as of 16:55, 19 March 2020
Leaky Chamber
Leaky chamber test
- When the leakage is so little that cannot be noticed in a short amount of time, we recommend to follow the Leaky chamber test below:
- Decrease the oxygen concentration down to ~ 50 μM (using Dithionite or N2 injection)
- Let the O2k record overnight with the chamber closed and illumination switched off
- The oxygen concentration should decrease overtime due to the O2 consumed by OroboPOS as it is shown in the figure. In this case, the chamber is assembled properly.
- If the oxygen concentration increases over time, a reassemble of the chamber is needed.
- Text in discussion for the page:
- To exclude the existence of an unnoticed leak from the chamber, we recommend to follow the Leaky chamber test below - especially after chamber reassembly:
- Decrease the oxygen concentration down to ~ 50 μM (using Dithionite or N2 injection).
- Record the O2k measurement overnight with the chamber closed and illumination switched off.
- The oxygen concentration should decrease overtime due to the O2 consumed by OroboPOS as it is shown in the figure below. In this case, the chamber is assembled properly.
- If the oxygen concentration increases over time, a reassemble of the chamber is needed.