Difference between revisions of "Talk:O2k-Chambers"
From Bioblast
(Created page with "== Leaky Chamber == === Leaky chamber test === :::* When the leakage is so little that cannot be noticed in a short amount of time, we recommend to follow the ''Leaky chamber...") |
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::::# Let the O2k record overnight with the [[closed chamber|chamber closed]] and illumination switched off | ::::# Let the O2k record overnight with the [[closed chamber|chamber closed]] and illumination switched off | ||
::::# The oxygen concentration should decrease overtime due to the O<sub>2</sub> consumed by [[OroboPOS]] as it is shown in the figure. In this case, the chamber is assembled properly. | ::::# The oxygen concentration should decrease overtime due to the O<sub>2</sub> consumed by [[OroboPOS]] as it is shown in the figure. In this case, the chamber is assembled properly. | ||
::::# If the oxygen concentration increases | ::::# If the oxygen concentration increases over time, a reassemble of the chamber is needed. | ||
[[File:Leaking_chamber_2020-02-03 P2-03.png|1500px]] | [[File:Leaking_chamber_2020-02-03 P2-03.png|1500px]] | ||
::::*'''Text in discussion for the page:''' | |||
::::* To exclude the existence of an unnoticed leak from the chamber, we recommend to follow the ''Leaky chamber test'' below - especially after chamber reassembly: | |||
::::# Decrease the oxygen concentration down to ~ 50 μM (using [[Dithionite]] or N<sub>2</sub> injection). | |||
::::# Record the O2k measurement overnight with the [[closed chamber|chamber closed]] and illumination switched off. | |||
::::# The oxygen concentration should decrease overtime due to the O<sub>2</sub> consumed by [[OroboPOS]] as it is shown in the figure below. In this case, the chamber is assembled properly. | |||
::::# If the oxygen concentration increases over time, a reassemble of the chamber is needed. | |||
Revision as of 15:51, 19 March 2020
Leaky Chamber
Leaky chamber test
- When the leakage is so little that cannot be noticed in a short amount of time, we recommend to follow the Leaky chamber test below:
- Decrease the oxygen concentration down to ~ 50 μM (using Dithionite or N2 injection)
- Let the O2k record overnight with the chamber closed and illumination switched off
- The oxygen concentration should decrease overtime due to the O2 consumed by OroboPOS as it is shown in the figure. In this case, the chamber is assembled properly.
- If the oxygen concentration increases over time, a reassemble of the chamber is needed.
- Text in discussion for the page:
- To exclude the existence of an unnoticed leak from the chamber, we recommend to follow the Leaky chamber test below - especially after chamber reassembly:
- Decrease the oxygen concentration down to ~ 50 μM (using Dithionite or N2 injection).
- Record the O2k measurement overnight with the chamber closed and illumination switched off.
- The oxygen concentration should decrease overtime due to the O2 consumed by OroboPOS as it is shown in the figure below. In this case, the chamber is assembled properly.
- If the oxygen concentration increases over time, a reassemble of the chamber is needed.
