Talk:O2k-Chambers

Leaky Chamber

Leaky chamber test

• When the leakage is so little that cannot be noticed in a short amount of time, we recommend to follow the Leaky chamber test below:

1. Decrease the oxygen concentration down to ~ 50 μM (using Dithionite or N₂ injection)
2. Let the O2k record overnight with the chamber closed and illumination switched off
3. The oxygen concentration should decrease overtime due to the O₂ consumed by OroboPOS as it is shown in the figure. In this case, the chamber is assembled properly.
4. If the oxygen concentration increases over time, a reassemble of the chamber is needed.

• Text in discussion for the page:

• To exclude the existence of an unnoticed leak from the chamber, we recommend performing the following Leaky chamber test - especially after chamber reassembly:

1. Decrease the oxygen concentration down to ~ 50 μM (using Dithionite or N₂ injection).
2. Record the O2k measurement overnight with the chamber closed and illumination switched off.
3. The oxygen concentration should decrease overtime due to the O₂ consumed by the OroboPOS as shown in the figure below.
4. If the oxygen concentration increases over time, a reassemblye of the chamber is needed.

CecattoC (talk) 15:52, 19 March 2020 (CET). So, it should be done every reassemble otherwise nobody will noticed that? How frequent you received cases with this or noticed leaky chamber in our lab? If the user can see the decrease of oxygen consumed by the sensor in an half and hour or an hour closed chamber run, they don't need to perform the leaky chamber test, right?