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| == While in home office mode ==
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| :::* [[User:Gnaigere|Gnaigere]] ([[User talk:Gnaigere|talk]]) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda '''analysis-linked''' O2k-Open Support cases during this time, and collect real-time operation cases for future releases.
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| :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Do you select the appropriate marks for the instrumental background correction when planning to apply them to a protocol with a high O<sub>2</sub> regime?'''
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| ::::::: To obtain an accurate O<sub>2</sub> background correction, you must select the marks that correspond to your range of experimental O<sub>2</sub> levels. - »[[Oxygen_flux_-_instrumental_background#Linear_regressions_values_.28intercept_-_a.C2.B0_and_the_slope_-_b.C2.B0.29_are_very_different_than_the_expected_values | O2 Background correction - troubleshooting]]«
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| :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Do you load the correct instrumental background correction accordingly to your range of experimental O<sub>2</sub> levels?'''
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| ::::::: See how to perform the instrumental background test. - »[[Flux / Slope| O2 background correction in DatLab]]« and »[[Oxygen_flux_-_instrumental_background | Instrumental oxygen background]]«
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| :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''Did you know that a humid O2k-environment and/or liquid spillage may lead to a high raw POS signal [V]?'''
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| ::::::: See how to clean and dry the electrical connections of the OroboPOS-Connector.-»[[OroboPOS-Connector#Cleaning_the_electrical_connections|Cleaning the electrical connections]]«
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| :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you know how the "switch components" approach can help you find what is causing your O2k to not reach the quality control specifications?'''
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| ::::::: Do not despair if specifications given in the O2k-Manuals are not obtained. See how to troubleshoot on your own.-»[[Oxygen_sensor_test#Switch_components_approach|Switch components approach]]«
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| == Removed for correction ==
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| :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''Do you perform the ''leaky chamber test'' after chamber assembly? '''
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| ::::::: - An unnoticed leak from the chamber may affect your data, therefore we recommend performing the ''Leaky chamber test'' - especially after chamber reassembly. - »[[Talk:O2k-Chamber_MitoPedia#Leaky_Chamber|Leaky chamber test]]«
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| :::::::» https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly
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| == In discussion ==
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| == O2k-Open Support alert circulars ==
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| === O2k-Open Support alert circular 2020(01): in prep ===
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| ::'''''New''''' [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.'''
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| ::::::: See how you can check that the two blue OroboPOS-Holders are intact. - »[[O2k-OroboPOS-Holder_MitoPedia#O2k-OroboPOS-Holder:_Leaky_chamber_due_to_damaged_OroboPOS-Holder |O2k-OroboPOS-Holder]]«
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| ::'''''New''''' [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Calibration parameters are transferred to experimental DatLab files'''
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| ::::::: Save calibration information from selected [[Marks - DatLab |Marks]] while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files. - »[[Oxygen_calibration_-_DatLab#Oxygen_calibration_in_instrumental_and_experimental_DatLab_files | Oxygen calibration - DatLab]]«
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| :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''Do you perform O2k-Chamber volume calibration?'''
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| ::::::: The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the [[O2k-sV-Module]]). - »[[O2k-Chamber MitoPedia]]«
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| :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you use the correct O<sub>2</sub> solubility factor (F<sub>M</sub>) in the ‘O2 calibration’ window in DatLab?
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| ::::::: The O<sub>2</sub> solubility factor varies according to the medium used and influences the air calibration. - »[[Oxygen solubility factor]]«
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