Talk:Oroboros FAQ

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OROBOROS FAQ

1. Q: What is the advantage of titration-injection micropump? Is manual pipetting to add sample to the chamber difficult? How is it different from ISS-integrated suction system and microsyringe set in O2K-core?

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A: TIP2k: Basically, the TIP2k makes life easier and work more comfortable, like automated determinations of oxygen background. If you are interested in e.g oxygen kinetics at low oxygen or very precise small step uncoupler titration, then the more advanced features of the TIP2k are necessary. If you want to use the O2k-Modules mentioned above were strongly recommend purchasing also the TIP2k. Most MultiSesnor measurements are also possible without it, but it gets dedious. For more information please have a look at the following link (http://www.oroboros.at/index.php?TIP2k). ISS: The ISS is for siphoning off excess medium from the O2k-Stopper and for emptying the O2k-Chambers. The ISS is included in the purchase of an O2k-Core and this suction pump is essential for the everyday usage of the instrument. The Microsyringe Set is another O2k-Core-accessory used for manual titrations.

2. Q: Are fluorescence sensors used for only green and blue emission? Is sensor not suitable for red emission? How about the excitation? Does the machine use a wide excitation spectrum?

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A: You are correct, there was a confusion of excitation and emmision on our webpage. I have corrected this on http://wiki.oroboros.at/index.php/O2k-Fluorescence_LED2-Module. Thank you for bringing this to our attention!. Please note that the applications currently envisaged are listed on the top of the linked page (under description). We do not use a wide exciation but separate LED sources for the two mentioned excitation colors.

3. Q:If only Ca+2 is detected with the suitable module? Or, other ions such Na+, phosphate, K+ can also be detected.

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A: I tried to answer your question here: http://wiki.oroboros.at/index.php/Talk:O2k-TPP%2B_and_Ca2%2B_ISE-Module

4. Q: Is special organ tissue or cell preparation needed to measure ions? Or, whole tissue and adherent/suspended cells are used.

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A: Adherent cells (without bringing them in suspension are conceptional not well suited for our system, see http://wiki.oroboros.at/index.php/Adherent_cells. TPP+ has been measured now with three types of samples, isolated mitochondria, permabilized cultures cells and permeabilized tissues. See http://wiki.oroboros.at/index.php/Mitochondrial_membrane_potential for an overview. We have comparable little experience with Ca2+, please see http://wiki.oroboros.at/index.php/Calcium_electrode. I do not see any reason that would exclude a type of sample here.

5. Q: Where do you get NO sensor and the cost of it?

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A: NO-Sensors: NO and H2O2 sensors use an amperometric measurement principle (Amp-Module), comparable to the polarographic oxygen sensor (POS), which yields an amperometric signal at a given polarization voltage. We supply custom-made stoppers that can accommodate such sensors. All amperometric sensors, like (H2S, H2O2) that have the same connection, can be used with the O2k. However, we have to mention that, while NO is measured by quite a few of our customers, the usability of any H2O2 electrode currently on the market is sometimes questioned. However, this depends on your sensitivity needs. We offer the O2k-MultiSensor Stoppers, for further information please check the following link: http://www.bioblast.at/index.php/O2k-NO_Amp-Module. For prices of NO electrodes (shaft with 2 mm diameter) please consult the manufacturer, e.g. WPI. WPI's 2 mm electrode is called "ISO-NOP" You will need the electrode housing , "replacement" sleeves #5436 (there are non with just the housing) and filling solution #7325. That is, unless they have changed the composition of their packages, so please inquire with them. For compatibility with our system the important point is the diameter of the sensor: 2mm.

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