Talk:TMPD: Difference between revisions

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=== Preparation of 200 mM stock ===
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TMPDΒ  (C<sub>10</sub>H<sub>16</sub>N<sub>2</sub>*2HCl): Sigma T 3134, 5 g, store at RT; FW = 237.2.
== Popular Bioblast page ==
[[TMPD]] has been accessed more than
::1) Weigh 47.4 mg TMPD
:* 10,000 times (2018-10-18)
::2) Dissolve in 1 ml H<sub>2</sub>O
:* 5,000 times (2015-05-24)
::3) Divide into 0.2 ml portions
::4) Protect from light!
::5) Store frozen at -20 Β°C
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<span style="color:#8B008B"> '''Caution:''' Light sensitive (store solution in a dark glass vial). Take care to minimize light and temperature exposure during use!</span>
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It is important to addΒ  [[Ascorbate]] before TMPDΒ  to avoidΒ  uncontrollable autoxidation.Β  Ascorbate maintains TMPD in aΒ  reducedΒ  state. Electrons flow from TMPD to cytochrome c.
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For preparation and titration please visit [[Ascorbate]]
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'''Oxygraph-2k Manual Titrations:''' [[MiPNet09.12]]
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::* Titration volume: 5 Β΅l using a 25 Β΅l syringe
::* Final conc. in 2 ml O2k-chamber: 0.5 mM

Latest revision as of 16:31, 18 October 2018

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