Template:Amp slope smoothing: Difference between revisions
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* Recommendation: Set "Slope smoothing" to 20 for performing and analyzing experiments with biological sample (strongly recommended in the case of uncoupler titrations). A slope smoothing of 20 increases the time or wavelength | * Recommendation: Set "Slope smoothing" of the Amp slope to 20 for performing and analyzing experiments with biological sample (strongly recommended in the case of uncoupler titrations). A slope smoothing of 20 increases the time or wavelength resolution, but increases the noise of the signal. | ||
:The following DatLab traces illustrate an example of "Slope smoothing" using 20 versus 40 data points: | :The following DatLab traces illustrate an example of "Slope smoothing" using 20 versus 40 data points: | ||
:::* '''Slope smoothing: 20''' | |||
[[File:Amp slope smoothing 20.png|1100px]] | [[File:Amp slope smoothing 20.png|1100px]] | ||
:::* '''Slope smoothing: 40''' | |||
[[File:Amp slope smoothing 40.png|1100px]] | [[File:Amp slope smoothing 40.png|1100px]] |
Latest revision as of 12:09, 4 March 2020
- Recommendation: Set "Slope smoothing" of the Amp slope to 20 for performing and analyzing experiments with biological sample (strongly recommended in the case of uncoupler titrations). A slope smoothing of 20 increases the time or wavelength resolution, but increases the noise of the signal.
- The following DatLab traces illustrate an example of "Slope smoothing" using 20 versus 40 data points:
- Slope smoothing: 20
- Slope smoothing: 40