Difference between revisions of "Template:BioblastAlert2021"
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=== Bioblast alert 2021(03) - ATP production PĀ»: 2021-01-29 === | |||
:{| | |||
| width="100" align="center" |[[File:MitoFit Preprints pdf.png|100px|link=https://wiki.oroboros.at/index.php/Cardoso_2021_MitoFit_MgG |MitoFit pdf]] | |||
|'''SUIT protocols with Magnesium Greenā¢ (MgG) can be used to study respiration and ATP production PĀ» '''<br> | |||
MgG dye does not affect respiration of cardiac isolated mitochondria.Ā <br> | |||
* Cardoso LHD, Doerrier C, Gnaiger E (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. MitoFit Preprints 2021.1. [https://wiki.oroboros.at/index.php/Cardoso_2021_MitoFit_MgG doi:10.26124/mitofit:2021-0001]. | |||
|} | |||
:{| | |||
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]] | |||
|'''Measuring ATP-ADP exchange using the fluorophore Magnesium Green''' <br> | |||
: from the O2k-Network Ā»[[HU Budapest Chinopoulos C]]Ā« <br> | |||
* Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48.Ā [[Chinopoulos 2014 Methods Enzymol|Ā»Bioblast linkĀ«]] | |||
|} | |||
:{| | |||
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]] | |||
| '''Magnesium Green and PĀ» ā which sample preparations work?'''<br> | |||
Magnesium Green (MgG) can be used with mitochondrial preparations: isolated mitochondria, tissue homogenates, and permeabilized cells and tissues. <br> | |||
- Ā»'''[[Magnesium_Green|MgG]]'''Ā« and Ā»'''[[Magnesium_Green#Publications:_MgG|Publications MgG]]'''Ā« <br> | |||
- communicated by Ā»[[Cardoso Luiza]]Ā« and Ā»[[Komlodi Timea]]Ā« | |||
|} | |||
:{| | |||
| width="110" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] | |||
|'''Did you know that you can measure the respiratory capacity available for PĀ» in living cells?'''<br> | |||
SUIT protocols from the SUIT-003 family guide you through the titrations to analyze [[R-L net ROUTINE capacity|''R-L'' net ROUTINE capacity]].<br> | |||
- Ā»'''[[SUIT-003 O2 ce D009]]'''Ā« and '''Ā»[[SUIT-003 O2 ce-pce D020]]'''Ā« <br> | |||
- communicated by Ā»[[Cardoso Luiza]]Ā« and Ā»[[Komlodi Timea]]Ā« | |||
|} | |||
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=== Bioblast alert 2021(02) - Permeabilized fibers: 2021-01-21 === | === Bioblast alert 2021(02) - Permeabilized fibers: 2021-01-21 === |
Revision as of 11:24, 29 January 2021
Bioblast alert 2021(03) - ATP production PĀ»: 2021-01-29
SUIT protocols with Magnesium Greenā¢ (MgG) can be used to study respiration and ATP production PĀ»
MgG dye does not affect respiration of cardiac isolated mitochondria.
- Cardoso LHD, Doerrier C, Gnaiger E (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. MitoFit Preprints 2021.1. doi:10.26124/mitofit:2021-0001.
Measuring ATP-ADP exchange using the fluorophore Magnesium Green
- from the O2k-Network Ā»HU Budapest Chinopoulos CĀ«
- Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. Ā»Bioblast linkĀ«
- from the O2k-Network Ā»HU Budapest Chinopoulos CĀ«
Magnesium Green and PĀ» ā which sample preparations work?
Magnesium Green (MgG) can be used with mitochondrial preparations: isolated mitochondria, tissue homogenates, and permeabilized cells and tissues.
- Ā»MgGĀ« and Ā»Publications MgGĀ«
- communicated by Ā»Cardoso LuizaĀ« and Ā»Komlodi TimeaĀ«
Did you know that you can measure the respiratory capacity available for PĀ» in living cells?
SUIT protocols from the SUIT-003 family guide you through the titrations to analyze R-L net ROUTINE capacity.
- Ā»SUIT-003 O2 ce D009Ā« and Ā»SUIT-003 O2 ce-pce D020Ā«
- communicated by Ā»Cardoso LuizaĀ« and Ā»Komlodi TimeaĀ«
Bioblast alert 2021(02) - Permeabilized fibers: 2021-01-21
A quote from MitoPathways Chapter 3. Normalization: Respiratory OXPHOS capacity P in human skeletal muscle ranges from 60 to 160 pmolāsĀ-1āmgĀ-1 based on wet muscle mass of permeabilized fibers. By comparison, mass-specific electron transfer capacity E is approximately 25 pmolās-1āmg-1 in platelets and senescent fibroblasts and 40 to 80 pmolās-1āmg-1 in PBMC, HEK, CEM, HUVEC, and young fibroblasts. ā Are these values comparable? - Ā»Gnaiger 2020 BEC MitoPathwaysĀ«.
Statistical evaluation on replica in high-resolution respirometry with permeabilized skeletal muscle fibers
- from the O2k-Network Ā»AU Melbourne Bishop DJĀ«
- Jacques M, Kuang J, Bishop DJ, Yan X, Alvarez-Romero J, Munson F, Garnham A, Papadimitriou I, Voisin S, Eynon N (2019) Mitochondrial respiration variability and simulations in human skeletal muscle: The Gene SMART study. FASEB J 34:2978-86. - Ā»Bioblast linkĀ«
- from the O2k-Network Ā»AU Melbourne Bishop DJĀ«
Effect of temperature on mitochondrial respiratory capacity of cardiac permeabilized fibers
- from the O2k-Network Ā»AT Innsbruck Gnaiger EĀ« , Ā»CA Rimouski Blier PUĀ« and Ā»CA Edmonton Lemieux HĀ«
- Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. Ā»Bioblast linkĀ«
What is the optimal ADP concentration to evaluate OXPHOS capacity in permeabilized muscle fibers (pfi)?
For the evaluation of OXPHOS capacity, the limitation of respiratory fluxes must be avoided by using a kinetically-saturating ADP concentration. In permeabilized muscle fibers, an ADP concentration of 5 mM or higher is needed to reach kinetically-saturating levels.
- Ā»Optimum ADP concentration in pfiĀ«
Bioblast alert 2021(01) - MitoPathways: the new BEC edition of the Blue Book: 2021-01-05
The 5th edition of MitoPathways features new chapters on additivity of mitochondrial pathway capacities and protonmotive pressure ā one step beyond āĪØ and the protonmotive force. - Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2: 112pp. doi:10.26124/bec:2020-0002. - Ā»Open AccessĀ«