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Difference between revisions of "Template:Current week ecosystem agenda"

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{{Template:Science for Ukraine}}
{{Template:Science for Ukraine}}
  2023 Week 51
  2024 Week 16
 
*'''Dec- 18 Mo'''
:::: '''»[[SBC 2023 Goa IN]]«''' Dec 18-20


* '''Apr-15 Mo'''
:{|
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|''' Why add azide after ascorbate and TMPD when measuring Complex IV activity? '''<br>
|'''Do you observe either a zero or low raw O<sub>2</sub> signal when starting a measurement?'''<br>
The O<sub><small>2</small></sub> flux measured after the titration of ascorbate and TMPD is due to both CIV activity and autooxidation of these compounds. By subsequently inhibiting CIV activity with azide, the O<sub><small>2</small></sub> flux due to autooxidation (chemical background) can be determined and subtracted from the initial measurement. <br>  
Perform a test to evaluate whether this is related to an improperly positioned chamber or a faulty connection between the OroboPOS and the OroboPOS-Connector. <br>
- »'''[[Complex IV]]'''«, »'''[[Chemical background]] '''«  <br>
- »'''[https://wiki.oroboros.at/index.php/Oxygen_signal#Raw_signal Oxygen signal]'''«<br>
- communicated by »[[Grings Mateus]]« and »[[Timon-Gomez Alba]]«  
- communicated by »[[Grings Mateus]]« and »[[Schmitt Sabine]]«
|}
|}
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*'''Dec- 19 Tu'''
* '''Apr-16 Tu'''
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* '''Apr-17 We'''  
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*'''Dec- 20 We'''
* '''Apr-18 Th'''  
:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|'''"SIRT4 [Sirtuin 4] knockdown increases mitochondrial capacity to oxidize fatty acids, decreasing LEAK respiration and further increasing mitochondrial elongation via its regulation of mitochondrial fusion."''' <br>
|''' How to store the POS overnight and up to several months? '''<br>
* Noone J, Rochfort KD, O'Sullivan F, O'Gorman DJ (2023) SIRT4 is a regulator of human skeletal muscle fatty acid metabolism influencing inner and outer mitochondrial membrane-mediated fusion. [[Noone 2023 Cell Signal|»Bioblast link«]]
With the POS still in place, fill the O2k-chamber and receptacle of the loosely inserted stopper with 70 % EtOH, add the Cover-slip and switch off the O2K. <br>
:from the O2k-Network »[[US FL Orlando Goodpaster BH]]« and »[[IE Dublin O Gorman D]]«
- »'''[[MiPNet19.03 O2k-cleaning and ISS]]'''«<br>
- communicated by »[[Leo Elettra]]« and »[[Grings Mateus]]«
|}
|}
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*'''Dec- 21 Th'''
* '''Apr-19 Fr'''
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*'''Dec- 22 Fr'''
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'''<big>Next Week</big>'''
'''<big>Next Week</big>'''
  2023 Week 52
  2024 Week 17


*'''Dec- 25 Mo'''
* '''Apr-22 Mo'''
:[[File:Christmas2023.jpg|500px]]
 
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*'''Dec- 26 Tu'''
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*'''Dec- 27 We'''
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
|'''Do you observe a high signal at zero oxygen?'''<br>
|'''How can I evaluate the effect of one specific titration of a complex SUIT protocol?'''<br>
If the quality of your dithionite solution has already been checked, perform a leak current test to evaluate which electrical component is generating the out-of-scale signal.  
Flux control efficiencies express the change in respiration after a single step, in which there is a change in a metabolic control variable.<br>
<br>
- »'''[[Flux control efficiency]]'''«, »'''[[Metabolic control variable]]'''«<br>
- »'''[https://wiki.oroboros.at/index.php/High_signal_at_zero_oxygen Leak current test]'''«<br>
- communicated by »[[Cardoso Luiza]]« and »[[Baglivo Eleonora]]«  
- communicated by »[[Baglivo Eleonora]]« and »[[Grings Mateus]]«
|}
|}
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*'''Dec- 28 Th'''
* '''Apr-23 Tu'''  
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*'''Dec- 29 Fr'''
* '''Apr-24 We'''
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* '''Apr-25 Th'''
:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''"Leptin receptor signaling in Schwann cells adjusts catabolic processes, including mitochondrial respiration and autophagy, facilitating nerve repair."''' <br>
|'''"Our results further enhance the understanding and relevance of bioenergetic function in liver ischemia and transplantation and provide the basis for further consideration of these parameters as biomarkers in LT [liver transplantation]."''' <br>
* Sundaram VK, Schütza V, Schröter NH, Backhaus A, Bilsing A, Joneck L, Seelbach A, Mutschler C, Gomez-Sanchez JA, Schäffner E, Sánchez EE, Akkermann D, Paul C, Schwagarus N, Müller S, Odle A, Childs G, Ewers D, Kungl T, Sitte M, Salinas G, Sereda MW, Nave KA, Schwab MH, Ost M, Arthur-Farraj P, Stassart RM, Fledrich R (2023) Adipo-glial signaling mediates metabolic adaptation in peripheral nerve regeneration. [[Sundaram_2023_Cell_Metab|»Bioblast link«]]
* Meszaros AT, Weissenbacher A, Schartner M, Egelseer-Bruendl T, Hermann M, Unterweger J, Mittelberger C, Reyer BA, Hofmann J, Zelger BG, Hautz T, Resch T, Margreiter C, Maglione M, Komlódi T, Ulmer H, Cardini B, Troppmair J, Öfner D, Gnaiger E, Schneeberger S, Oberhuber R (2024) The predictive value of graft viability and bioenergetics testing towards the outcome in liver transplantation. [[Meszaros_2024_Transpl_Int|»Bioblast link«]]
:from the O2k-Network »[[DE_Leipzip_Ost_M]]«
:from the O2k-Network »[[AT Innsbruck Schneeberger S]] and [[AT Innsbruck Oroboros]]«
|}
|}  
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* '''Apr-26 Fr'''
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Revision as of 14:02, 15 April 2024

Gentle Science
Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. -  »More details
A future for Ukraine is the future of its science
2024 Week 16
  • Apr-15 Mo
O2k-Open SupportInfo: improve Do you observe either a zero or low raw O2 signal when starting a measurement?

Perform a test to evaluate whether this is related to an improperly positioned chamber or a faulty connection between the OroboPOS and the OroboPOS-Connector.
- »Oxygen signal«
- communicated by »Grings Mateus« and »Schmitt Sabine«


  • Apr-16 Tu

  • Apr-17 We

  • Apr-18 Th
O2k-Open SupportInfo: improve How to store the POS overnight and up to several months?

With the POS still in place, fill the O2k-chamber and receptacle of the loosely inserted stopper with 70 % EtOH, add the Cover-slip and switch off the O2K.
- »MiPNet19.03 O2k-cleaning and ISS«
- communicated by »Leo Elettra« and »Grings Mateus«


  • Apr-19 Fr

Next Week

2024 Week 17
  • Apr-22 Mo
MitoPedia
How can I evaluate the effect of one specific titration of a complex SUIT protocol?

Flux control efficiencies express the change in respiration after a single step, in which there is a change in a metabolic control variable.
- »Flux control efficiency«, »Metabolic control variable«
- communicated by »Cardoso Luiza« and »Baglivo Eleonora« 


  • Apr-23 Tu

  • Apr-24 We

  • Apr-25 Th
O2k-Publications in the MiPMap "Our results further enhance the understanding and relevance of bioenergetic function in liver ischemia and transplantation and provide the basis for further consideration of these parameters as biomarkers in LT [liver transplantation]."
  • Meszaros AT, Weissenbacher A, Schartner M, Egelseer-Bruendl T, Hermann M, Unterweger J, Mittelberger C, Reyer BA, Hofmann J, Zelger BG, Hautz T, Resch T, Margreiter C, Maglione M, Komlódi T, Ulmer H, Cardini B, Troppmair J, Öfner D, Gnaiger E, Schneeberger S, Oberhuber R (2024) The predictive value of graft viability and bioenergetics testing towards the outcome in liver transplantation. »Bioblast link«
from the O2k-Network »AT Innsbruck Schneeberger S and AT Innsbruck Oroboros«

  • Apr-26 Fr