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2022 Week 31
{{Template:Science for Ukraine}}
{{Template:Science for Ukraine}}
*'''Aug-01 Mo'''  
 
[[Image:O2k-Workshops.png|60px|link=MiPNet27.03 IOC154 Innsbruck AT]]  <big>'''[[MiPNet27.03 IOC154 Innsbruck AT|154th Oroboros O2k-Coaching Days - Advanced and NextGen-O2k]]'''</big> Aug 1-5
2024 Week 19
 
* '''May-06 Mo'''
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
|''' O2k-checklist: Get started with an O2k-experiment''' <br>
|''' What is the effective concentration of digitonin for complete plasma membrane permeabilization?'''<br>
The O2k-checklist provides an overview of all essential steps for starting an O2k-experiment.
It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane. <br>
<br>
- »'''[[Digitonin]]'''«, »'''[[SUIT-010]]'''« <br>
- » '''[[MiPNet20.04_O2k-checklist]]'''« <br>
- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«  
- » communicated by »[[Cecatto Cristiane]]« and »[[Schmitt Sabine]]«
|}
|}
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*'''Aug-02 Tu'''  
* '''May-07 Tu'''  
[[Image:O2k-Workshops.png|60px|link=MiPNet27.03 IOC154 Innsbruck AT]]  <big>'''[[MiPNet27.03 IOC154 Innsbruck AT|154th Oroboros O2k-Coaching Days - Advanced and NextGen-O2k]]'''</big> Aug 1-5
:{|
:{|
| width="110" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]  
| width="110" align="center" | [[File:Tributes to pioneers icon.jpg|left|80px|BEC Tributes to pioneers in bioenergetics|link=Tributes to pioneers in bioenergetics]]
|''' What are the substrate-uncoupler-inhibitor titration reference protocols?'''<br>
|'''Tributes to pioneers in bioenergetics''' will provide a fresh look at concepts in a state of flow rather than as fixed paradigms, which tend to be forgotten or distorted without wandering in the garden of memories.<br>
These protocols provide a common baseline for comparison of mitochondrial respiratory control in a large variety of species, tissues and cell types, mt-preparations and laboratories.
- »'''[[Tributes to pioneers in bioenergetics |Tributes to pioneers in bioenergetics - wandering in the garden of memories]]'''« - a new BEC Publication Series<br>
<br>
- »''' [[SUIT reference protocol]]'''«
 
- communicated by »[[Cardoso Luiza HD]]« and »[[Tindle-Solomon Lisa]]«
|}
|}
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*'''Aug-03 We'''
* '''May-08 We'''  
[[Image:O2k-Workshops.png|60px|link=MiPNet27.03 IOC154 Innsbruck AT]]  <big>'''[[MiPNet27.03 IOC154 Innsbruck AT|154th Oroboros O2k-Coaching Days - Advanced and NextGen-O2k]]'''</big> Aug 1-5
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* '''May-09 Th'''  
:{|
:{|
 
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="110" align="center" | [[File:PlayVideo.jpg|left|80px|O2k-Videosupport|link=O2k-Videosupport]]
|'''"The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"''' <br>
 
* Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem [[Vera-Vives 2024 Plant Physiol Biochem|»Bioblast link«]]
|''' O<sub>2</sub> flux analysis with DatLab 7.4'''<br>
:from the O2k-Network »[[IT Padova Morosinotto T]
Use the O<sub>2</sub> analysis template:  <br>
 
- »'''[https://www.youtube.com/watch?v=HRAGvmaK01I%7C O2k-Videosupport: O<sub>2</sub> flux analysis with DatLab 7.4]'''«<br>
|}
|}
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* '''May-10 Fr'''
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'''<big>Next Week</big>'''


*'''Aug-04 Th'''
  2024 Week 20
[[Image:O2k-Workshops.png|60px|link=MiPNet27.03 IOC154 Innsbruck AT]] <big>'''[[MiPNet27.03 IOC154 Innsbruck AT|154th Oroboros O2k-Coaching Days - Advanced and NextGen-O2k]]'''</big> Aug 1-5


* '''May-13 Mo'''
:{|
:{|
 
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
|'''Do you see fluctuations of O<sub>2</sub> slope negative following a pattern similar to the Peltier power, but without block temperature variation?'''<br>
 
Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O<sub>2</sub> slope negative.
|''' Why do we perform multiple H<sub>2</sub>O<sub>2</sub> calibrations in the Amplex® UltraRed assay?'''<br>
<br>
The sensitivity to H<sub>2</sub>O<sub>2</sub> of the fluorometric signal changes during the experiment as a function of: (''1'') experimental time and accumulating UltroxRed (similar to resorufin), (''2'') changes of the optical properties due to titrations, and (''3'') the radical scavenging capacity of the sample. Therefore, H<sub>2</sub>O<sub>2</sub> calibrations are performed before and after sample addition, and after selected titration steps.  <br>
- »'''[[Oxygen_flux_-_instrumental_background#Fluctuations_of_O2_slope_negative|Fluctuations of O<sub>2</sub> slope negative]]'''«<br>
 
- communicated by »[[Cardoso Luiza]]« and »[[Grings Mateus]]«
- »'''[[Amplex UltraRed]]'''« and »'''[[Komlodi_2021_BEC_AmR-O2 |Komlodi et al BEC 2021.4]]'''«<br>
|}
|}
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* '''May-14 Tu'''  
*'''Aug-05 Fr'''
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[[Image:O2k-Workshops.png|60px|link=MiPNet27.03 IOC154 Innsbruck AT]]  <big>'''[[MiPNet27.03 IOC154 Innsbruck AT|154th Oroboros O2k-Coaching Days - Advanced and NextGen-O2k]]'''</big> Aug 1-5
* '''May-15 We'''  
 
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* '''May-16 Th'''
:{|
:{|
 
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
| width="110" align="center" | [[File:PlayVideo.jpg|left|80px|O2k-Videosupport|link=O2k-Videosupport]]
|''' "The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."'''<br>
 
- »'''[[MiPNet27.07 Sample Holder]]'''«<br>
|'''Watch these videos to get started with the Q-Module'''<br>
- communicated by »[[Willis Jaime R]]« and »[[Timon-Gomez Alba]]«
 
- »'''[https://wiki.oroboros.at/index.php/O2k-Videosupport#Q-Module_.28NextGen-O2k_-_DatLab_7.29 O2k-Videosupport:Q-Module]'''«<br>
|}
|}
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* '''May-17 Fr'''
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Revision as of 16:15, 6 May 2024

Gentle Science
Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. -  »More details
A future for Ukraine is the future of its science
2024 Week 19
  • May-06 Mo
MitoPedia
What is the effective concentration of digitonin for complete plasma membrane permeabilization?

It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane.
- »Digitonin«, »SUIT-010« 
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba« 


  • May-07 Tu
BEC Tributes to pioneers in bioenergetics
Tributes to pioneers in bioenergetics will provide a fresh look at concepts in a state of flow rather than as fixed paradigms, which tend to be forgotten or distorted without wandering in the garden of memories.

- »Tributes to pioneers in bioenergetics - wandering in the garden of memories« - a new BEC Publication Series


  • May-08 We

  • May-09 Th
O2k-Publications in the MiPMap "The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"
  • Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem »Bioblast link«
from the O2k-Network »IT Padova Morosinotto T«

  • May-10 Fr

Next Week

2024 Week 20
  • May-13 Mo
O2k-Open SupportInfo: take care Do you see fluctuations of O2 slope negative following a pattern similar to the Peltier power, but without block temperature variation?

Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O2 slope negative.
- »Fluctuations of O2 slope negative«
- communicated by »Cardoso Luiza« and »Grings Mateus«


  • May-14 Tu

  • May-15 We

  • May-16 Th
O2k-Open SupportInfo: improve "The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."

- »MiPNet27.07 Sample Holder«
- communicated by »Willis Jaime R« and »Timon-Gomez Alba«


  • May-17 Fr

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