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Revision as of 16:15, 6 May 2024

Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. - »More details

-» A future for Ukraine is the future of its science

2024 Week 19

May-06 Mo

What is the effective concentration of digitonin for complete plasma membrane permeabilization?

It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane.
- »Digitonin«, »SUIT-010«
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba«

May-07 Tu

Tributes to pioneers in bioenergetics will provide a fresh look at concepts in a state of flow rather than as fixed paradigms, which tend to be forgotten or distorted without wandering in the garden of memories.

- »Tributes to pioneers in bioenergetics - wandering in the garden of memories« - a new BEC Publication Series

May-08 We

May-09 Th

"The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"

Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem »Bioblast link«

from the O2k-Network »IT Padova Morosinotto T«

May-10 Fr

Next Week

2024 Week 20

May-13 Mo

Do you see fluctuations of O₂ slope negative following a pattern similar to the Peltier power, but without block temperature variation?

Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O₂ slope negative.
- »Fluctuations of O₂ slope negative«
- communicated by »Cardoso Luiza« and »Grings Mateus«

May-14 Tu

May-15 We

May-16 Th

"The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."

- »MiPNet27.07 Sample Holder«
- communicated by »Willis Jaime R« and »Timon-Gomez Alba«

May-17 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 43
+Week 19
-*'''Oct- 23 Mo'''
+* '''May-06 Mo'''
 :{|
-| width="110" align="center" | [[Image:O2k-Feedback.jpg|60px|link=https://wiki.oroboros.at/index.php/O2k-Feedback |O2k-Feedback]]
+| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|''' ''“Thanks for this excellent guidance and support thus far.”'''''<br>
+|''' What is the effective concentration of digitonin for complete plasma membrane permeabilization?'''<br>
-[[Maarman Gerald J|Maarman Gerald J]], from the O2k-Network Lab »[[ZA Cape Town Maarman GJ]]«<br>
+It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane. <br>
-- »'''[[O2k-Feedback]]'''«<br>
+- »'''[[Digitonin]]'''«, »'''[[SUIT-010]]'''« <br>
+- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«
 |}
 ----
-*'''Oct- 24 Tu'''
+* '''May-07 Tu'''
 :{|
-| width="100" align="center" |[[File:MitoFit Preprints.png|100px|link=https://wiki.oroboros.at/images/e/eb/Garcia_Menendez_2023_MitoFit.pdf |MitoFit pdf]]
+| width="110" align="center" | [[File:Tributes to pioneers icon.jpg|left|80px|BEC Tributes to pioneers in bioenergetics|link=Tributes to pioneers in bioenergetics]]
-|'''A theoretical communication from Garcia Menendez on electromagnetic coupling hypothesis.'''<br>
+|'''Tributes to pioneers in bioenergetics''' will provide a fresh look at concepts in a state of flow rather than as fixed paradigms, which tend to be forgotten or distorted without wandering in the garden of memories.<br>
-* Garcia Menendez R (2023) Update and a new statement of the electromagnetic coupling hypothesis to explain the translocation of protons in mitochondria, bacteria and chloroplasts. MitoFit Preprints 2023.8. https://doi.org/10.26124/mitofit:2023-0008
+- »'''[[Tributes to pioneers in bioenergetics |Tributes to pioneers in bioenergetics - wandering in the garden of memories]]'''« - a new BEC Publication Series<br>
 |}
 ----
-*'''Oct- 25 We'''
+* '''May-08 We'''
 ----
-*'''Oct- 26 Th'''
+* '''May-09 Th'''
 :{|
 | width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''"In collegiate swimmers, (…) a maximal exercise bout (i.e., the bucket test) was associated with minimal changes in ''cellular-level'' ''I''O<sub>2</sub> [oxygen flow in isolated PBMCs]. In contrast, due to the robust mobilization of immune cells in response to the maximal exercise bout, the ''tissue-level'' IO''<sub>2-Tissue</sub>'' [blood] was significantly elevated."''' <br>
+|'''"The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"''' <br>
-* Stampley JE, Cho E, Wang H, Theall B, Johannsen NM, Spielmann G, Irving BA (2023) Impact of maximal exercise on immune cell mobilization and bioenergetics. [[Stampley 2023 Physiol Rep|»Bioblast link«]]
+* Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem [[Vera-Vives 2024 Plant Physiol Biochem|»Bioblast link«]]
-:from the O2k-Network »[[US LA Baton Rouge Irving BA]]«
+:from the O2k-Network »[[IT Padova Morosinotto T]]«
 |}
 ----
-*'''Oct- 27 Fr'''
+* '''May-10 Fr'''
 ----
 '''<big>Next Week</big>'''
-Week 44
+Week 20
-*'''Oct- 30 Mo'''
+* '''May-13 Mo'''
-----
-*'''Oct- 31 Tu'''
 :{|
-| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
-|''' How to interpret cytochrome ''c'' efficiency '''<br>
+|'''Do you see fluctuations of O<sub>2</sub> slope negative following a pattern similar to the Peltier power, but without block temperature variation?'''<br>
-Stimulation of OXPHOS or ET capacity upon addition of cytochrome ''c''  provides evidence of cytochrome ''c'' loss, indicating damage of the mitochondrial outer membrane, which could be caused by sample preparation or pathological conditions. <br>
+Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O<sub>2</sub> slope negative.
-- »'''[https://wiki.oroboros.at/index.php/Cytochrome_c_control_efficiency Cytochrome ''c'' control efficiency]'''« <br>
+<br>
-- communicated by »[[Schmitt Sabine]]« and »[[Leo Elettra]]«
+- »'''[[Oxygen_flux_-_instrumental_background#Fluctuations_of_O2_slope_negative|Fluctuations of O<sub>2</sub> slope negative]]'''«<br>
+- communicated by »[[Cardoso Luiza]]« and »[[Grings Mateus]]«
 |}
 ----
-*'''Nov- 01 We'''
+* '''May-14 Tu'''
 ----
-*'''Nov- 02 Th'''
+* '''May-15 We'''
 ----
-*'''Nov- 03 Fr'''
+* '''May-16 Th'''
 :{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''"Peripheral impairments at the skeletal muscle level, namely reduced mitochondrial function and the presence of markers of impaired mitochondrial biogenesis emerged as major determinants of exercise intolerance and fatigue [in patients with post-acute sequelae of SARS-CoV-2 infection]"''' <br>
+|''' "The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."'''<br>
-* Colosio M, Brocca L, Gatti M, Neri M, Crea E, Cadile F, Canepari M, Pellegrino MA, Polla B, Porcelli S, Bottinelli R (2023) Structural and functional impairments of skeletal muscle in patients with post-acute sequelae of SARS-CoV-2 infection. [[Colosio 2023 J Appl Physiol (1985)|»Bioblast link«]]
+- »'''[[MiPNet27.07 Sample Holder]]'''«<br>
+- communicated by »[[Willis Jaime R]]« and »[[Timon-Gomez Alba]]«
 |}
+----
+* '''May-17 Fr'''
 ----