Template:Current week ecosystem agenda: Difference between revisions

Revision as of 16:15, 6 May 2024

Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. - »More details

-» A future for Ukraine is the future of its science

2024 Week 19

May-06 Mo

What is the effective concentration of digitonin for complete plasma membrane permeabilization?

It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane.
- »Digitonin«, »SUIT-010«
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba«

May-07 Tu

Tributes to pioneers in bioenergetics will provide a fresh look at concepts in a state of flow rather than as fixed paradigms, which tend to be forgotten or distorted without wandering in the garden of memories.

- »Tributes to pioneers in bioenergetics - wandering in the garden of memories« - a new BEC Publication Series

May-08 We

May-09 Th

"The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"

Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem »Bioblast link«

from the O2k-Network »IT Padova Morosinotto T«

May-10 Fr

Next Week

2024 Week 20

May-13 Mo

Do you see fluctuations of O₂ slope negative following a pattern similar to the Peltier power, but without block temperature variation?

Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O₂ slope negative.
- »Fluctuations of O₂ slope negative«
- communicated by »Cardoso Luiza« and »Grings Mateus«

May-14 Tu

May-15 We

May-16 Th

"The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."

- »MiPNet27.07 Sample Holder«
- communicated by »Willis Jaime R« and »Timon-Gomez Alba«

May-17 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 47
-*'''Nov- 20 Mo'''
+Week 19
+* '''May-06 Mo'''
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
+| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|'''Do I need to change the filter set of the O2k-Fluo Smart-Module?'''<br>
+|''' What is the effective concentration of digitonin for complete plasma membrane permeabilization?'''<br>
-Because two different filter sets can be used with the blue Fluo-Sensors, filters should be changed according to the fluorophore that will be used. Extra filters are provided for replacement in case of damage.
+It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane. <br>
-<br>
+- »'''[[Digitonin]]'''«, »'''[[SUIT-010]]'''« <br>
-- »'''[[MiPNet28.09_O2k-Fluo_Smart-Module_manual|O2k-Fluo Smart-Module manual]]'''« and »'''[[O2k-Fluo_Smart-Module#Fluorescence_dyes_and_settings|O2k-Fluo Smart-Module - Fluorescence dyes and settings]]'''« <br>
+- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«
-- communicated by »[[Cardoso Luiza]]« and »[[Timon-Gomez Alba]]«
 |}
 ----
-*'''Nov- 21 Tu'''
+* '''May-07 Tu'''
-----
-*'''Nov- 22 We'''
 :{|
-| width="100" align="center" |[[File:2023.4.jpg|right|150px|link=https://doi.org/10.26124/bec:2023-0004| Oliveira 2023 BEC]]
+| width="110" align="center" | [[File:Tributes to pioneers icon.jpg|left|80px|BEC Tributes to pioneers in bioenergetics|link=Tributes to pioneers in bioenergetics]]
-|'''“The cautionary note provided by these studies emphasizes the need for careful consideration of which probes and conditions can be used when assessing mitochondrial physiology in ''Drosophila''.”''' <br>
+|'''Tributes to pioneers in bioenergetics''' will provide a fresh look at concepts in a state of flow rather than as fixed paradigms, which tend to be forgotten or distorted without wandering in the garden of memories.<br>
-* Oliveira MF, Aveiro Y, Couto-Lima CA (2023) A navigation chart to avoid turbulence in ''Drosophila'' mitochondrial research. Bioenerg Commun 2023.4. https://doi.org/10.26124/bec:2023-0004
+- »'''[[Tributes to pioneers in bioenergetics |Tributes to pioneers in bioenergetics - wandering in the garden of memories]]'''« - a new BEC Publication Series<br>
-:from the O2k-Network »[[BR Rio de Janeiro Oliveira MF]]«
 |}
 ----
-*'''Nov- 23 Th'''
+* '''May-08 We'''
+----
+* '''May-09 Th'''
 :{|
 | width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''"Thoughtful design and execution of O2k experiments result in data that become a powerhouse for addressing many mitochondrial respiration research questions. "''' <br>
+|'''"The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"''' <br>
-* Walsh MA, Musci RV, Jacobs RA, Hamilton KL (2023)  A practical perspective on how to develop, implement, execute, and reproduce high-resolution respirometry experiments: The physiologist's guide to an Oroboros O2k [[Walsh 2023 FASEB J|»Bioblast link«]]
+* Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem [[Vera-Vives 2024 Plant Physiol Biochem|»Bioblast link«]]
-:from the O2k-Network »[[US CO Colorado Springs Jacobs RA]]« and »[[US CO Fort Collins Hamilton K]]«
+:from the O2k-Network »[[IT Padova Morosinotto T]]«
 |}
 ----
-*'''Nov- 24 Fr'''
+* '''May-10 Fr'''
 ----
+'''<big>Next Week</big>'''
-'''<big>Next Week</big>'''
+Week 20
-Week 48
-*'''Nov- 27 Mo'''
+* '''May-13 Mo'''
-----
-*'''Nov- 28 Tu'''
 :{|
-| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
-|'''Protocols that SUIT your research question'''<br>
+|'''Do you see fluctuations of O<sub>2</sub> slope negative following a pattern similar to the Peltier power, but without block temperature variation?'''<br>
-Using '''S'''ubstrate-'''U'''ncoupler-'''I'''nhibitor-'''T'''itration protocols you can study mitochondrial respiratory control through a sequence of coupling and pathway control states induced by multiple titrations within a single experimental assay. <br>
+Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O<sub>2</sub> slope negative.
-- »'''[https://wiki.oroboros.at/index.php/MitoPedia:_SUIT SUIT protocols]'''« and »'''[https://bioblast.at/index.php/SUITbrowser SUITbrowser]'''« <br>
+<br>
-- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«
+- »'''[[Oxygen_flux_-_instrumental_background#Fluctuations_of_O2_slope_negative|Fluctuations of O<sub>2</sub> slope negative]]'''«<br>
+- communicated by »[[Cardoso Luiza]]« and »[[Grings Mateus]]«
 |}
 ----
-*'''Nov- 29 We'''
+* '''May-14 Tu'''
 ----
-*'''Nov- 30 Th'''
+* '''May-15 We'''
 ----
-*'''Dec- 01 Fr'''  <span style="color:red">World AIDS day</span>
+* '''May-16 Th'''
 :{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''"We therefore hypothesized that the susceptibility to HIV-1 infection of FOXO1-inhibited resting T cells could be due to an increased cell metabolism. (...) Results obtained by high-resolution respirometry experiments of [human peripheral blood T cells] PBT (...) showed that [ROUTINE] respiration at the steady state was increased by [FOXO1 inhibitor] AS1842856."''' <br>
+|''' "The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."'''<br>
-* Roux A, Leroy H, De Muylder B, Bracq L, Oussous S, Dusanter-Fourt I, Chougui G, Tacine R, Randriamampita C, Desjardins D, Le Grand R, Bouillaud F, Benichou S, Margottin-Goguet F, Cheynier R, Bismuth G, Mangeney M (2019) FOXO1 transcription factor plays a key role in T cell-HIV-1 interaction.  [[Roux 2019 PLoS Pathog|»Bioblast link«]]
+- »'''[[MiPNet27.07 Sample Holder]]'''«<br>
-:from the O2k-Network »[[FR Paris Bouillaud F]]«
+- communicated by »[[Willis Jaime R]]« and »[[Timon-Gomez Alba]]«
 |}
+----
+* '''May-17 Fr'''
 ----