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Revision as of 16:15, 6 May 2024

Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. - »More details

-» A future for Ukraine is the future of its science

2024 Week 19

May-06 Mo

What is the effective concentration of digitonin for complete plasma membrane permeabilization?

It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane.
- »Digitonin«, »SUIT-010«
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba«

May-07 Tu

Tributes to pioneers in bioenergetics will provide a fresh look at concepts in a state of flow rather than as fixed paradigms, which tend to be forgotten or distorted without wandering in the garden of memories.

- »Tributes to pioneers in bioenergetics - wandering in the garden of memories« - a new BEC Publication Series

May-08 We

May-09 Th

"The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"

Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem »Bioblast link«

from the O2k-Network »IT Padova Morosinotto T«

May-10 Fr

Next Week

2024 Week 20

May-13 Mo

Do you see fluctuations of O₂ slope negative following a pattern similar to the Peltier power, but without block temperature variation?

Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O₂ slope negative.
- »Fluctuations of O₂ slope negative«
- communicated by »Cardoso Luiza« and »Grings Mateus«

May-14 Tu

May-15 We

May-16 Th

"The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."

- »MiPNet27.07 Sample Holder«
- communicated by »Willis Jaime R« and »Timon-Gomez Alba«

May-17 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 50
-*'''Dec- 11 Mo''' - International Mountain Day
+Week 19
+* '''May-06 Mo'''
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
+| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|'''Mountain slopes can be difficult, but O<sub>2</sub> slopes can be smooth with DatLab'''<br>
+|''' What is the effective concentration of digitonin for complete plasma membrane permeabilization?'''<br>
-The default setting of Slope smoothing is 40 data points, corresponding to an interval of 80 s at a data recording interval of 2 s. DatLab 7 expresses it in data points and DatLab 8 in seconds.
+It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane. <br>
-<br>
+- »'''[[Digitonin]]'''«, »'''[[SUIT-010]]'''« <br>
-- »'''[https://wiki.oroboros.at/index.php/Flux_/_Slope#Smoothing.2C_noise.2C_and_sample_stability Smoothing in Flux/Slope]'''«<br>
+- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«
-- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
 |}
 ----
-*'''Dec- 12 Tu'''
+* '''May-07 Tu'''
 :{|
-| width="100" align="center" |[[File:MitoFit Preprints.png|100px|MitoFit pdf]]
+| width="110" align="center" | [[File:Tributes to pioneers icon.jpg|left|80px|BEC Tributes to pioneers in bioenergetics|link=Tributes to pioneers in bioenergetics]]
-| '''From preprint to Gold Open Access publication in J Biol Chem'''<br>
+|'''Tributes to pioneers in bioenergetics''' will provide a fresh look at concepts in a state of flow rather than as fixed paradigms, which tend to be forgotten or distorted without wandering in the garden of memories.<br>
-* Gnaiger E (2023) Complex II ambiguities ― FADH2 in the electron transfer system. [[Gnaiger 2023 J Biol Chem|»Bioblast link«]]
+- »'''[[Tributes to pioneers in bioenergetics |Tributes to pioneers in bioenergetics - wandering in the garden of memories]]'''« - a new BEC Publication Series<br>
 |}
 ----
-*'''Dec- 13 We'''
+* '''May-08 We'''
 ----
-*'''Dec- 14 Th'''
+* '''May-09 Th'''
 :{|
 | width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''"HCR [High capacity runner rats] showed a consistent and robust trend of higher maximal oxygen consumption compared to LCR [low capacity runner rats], especially for long chain FAs [fatty acids]."''' <br>
+|'''"The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"''' <br>
-* Fleischman JY, Van den Bergh F, Collins NL, Bowers M, Beard DA, Burant CF (2023) Higher mitochondrial oxidative capacity is the primary molecular differentiator in muscle of rats with high and low intrinsic cardiorespiratory fitness. [[Fleischman 2023 Mol Metab|»Bioblast link«]]
+* Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem [[Vera-Vives 2024 Plant Physiol Biochem|»Bioblast link«]]
-:from the O2k-Network »[[US MI Ann Arbor Beard DA]]«
+:from the O2k-Network »[[IT Padova Morosinotto T]]«
 |}
 ----
-*'''Dec- 15 Fr'''
+* '''May-10 Fr'''
 ----
 '''<big>Next Week</big>'''
-Week 51
-*'''Dec- 18 Mo'''
+Week 20
-:::: '''»[[SBC 2023 Goa IN]]«''' Dec 18-20
+* '''May-13 Mo'''
 :{|
-| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
-|''' Why add azide after ascorbate and TMPD when measuring Complex IV activity? '''<br>
+|'''Do you see fluctuations of O<sub>2</sub> slope negative following a pattern similar to the Peltier power, but without block temperature variation?'''<br>
-The O<sub><small>2</small></sub> flux measured after the titration of ascorbate and TMPD is due to both CIV activity and autooxidation of these compounds. By subsequently inhibiting CIV activity with azide, the O<sub><small>2</small></sub> flux due to autooxidation (chemical background) can be determined and subtracted from the initial measurement. <br>
+Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O<sub>2</sub> slope negative.
-- »'''[[Complex IV]]'''«, »'''[[Chemical background]] '''«  <br>
+<br>
-- communicated by »[[Grings Mateus]]« and »[[Timon-Gomez Alba]]«
+- »'''[[Oxygen_flux_-_instrumental_background#Fluctuations_of_O2_slope_negative|Fluctuations of O<sub>2</sub> slope negative]]'''«<br>
+- communicated by »[[Cardoso Luiza]]« and »[[Grings Mateus]]«
 |}
 ----
-*'''Dec- 19 Tu'''
+* '''May-14 Tu'''
 ----
-*'''Dec- 20 We'''
+* '''May-15 We'''
+----
+* '''May-16 Th'''
 :{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''"SIRT4 [Sirtuin 4] knockdown increases mitochondrial capacity to oxidize fatty acids, decreasing LEAK respiration and further increasing mitochondrial elongation via its regulation of mitochondrial fusion."''' <br>
+|''' "The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."'''<br>
-* Noone J, Rochfort KD, O'Sullivan F, O'Gorman DJ (2023) SIRT4 is a regulator of human skeletal muscle fatty acid metabolism influencing inner and outer mitochondrial membrane-mediated fusion. [[Noone 2023 Cell Signal|»Bioblast link«]]
+- »'''[[MiPNet27.07 Sample Holder]]'''«<br>
-:from the O2k-Network »[[US FL Orlando Goodpaster BH]]« and »[[IE Dublin O Gorman D]]«
+- communicated by »[[Willis Jaime R]]« and »[[Timon-Gomez Alba]]«
 |}
 ----
-*'''Dec- 21 Th'''
+* '''May-17 Fr'''
-----
-*'''Dec- 22 Fr'''
 ----