Template:Current week ecosystem agenda: Difference between revisions

Latest revision as of 09:37, 21 May 2024

Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. - »More details

-» A future for Ukraine is the future of its science

2024 Week 21

May-20 Mo

How do I determine the optimal oligomycin concentration for my assay?

Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity.
- »Oligomycin titration test«
- communicated by »Baglivo Eleonora« and »Schmitt Sabine«

May-21 Tu

May-22 We - International Day for Biological Diversity

"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."

Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. »Bioblast link«

from the O2k-Network »NO Tromsoe Wood SH«

May-23 Th

May-24 Fr

Next Week

2024 Week 22

May-27 Mo

Same experimental conditions, but different O₂ fluxes in the left and right chambers?

Check for instrumental and operational factors that could be causing O₂ flux differences between the O2k-chambers.
- »Different O2 fluxes in left and right chamber«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-28 Tu

May-29 We

May-30 Th

"Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"

- »MiPNet20.06 Isolation mouse heart mitochondria«
- communicated by »Cardoso Luiza« and »Leo Elettra«

May-31 Fr

@@ Line 1: / Line 1: @@
-* '''Mar-23 Mo'''
+{{Template:Science for Ukraine}}
-:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you know that you can use the [[TIP2k-Module|Titration-Injection microPump (TIP2k)]] to perform an instrumental O<sub>2</sub> background on the [[O2k-sV-Module]]?'''
-::::::: '''(1) By adjusting the [[Dithionite|dithionite]] concentration to  2.5 mM''', the instrumental O<sub>2</sub> background can be performed with the "BG Feedback" settings. - »'''[[MiPNet24.14 O2k-sV-Module manual|O2k-sV-Module]]'''«
-::::::: '''(2) By adjusting the TIP2k setup with specific settings for the O2k-sV-Module''', the instrumental O<sub>2</sub> background can be performed in the O2k-sV-Module. - »'''[[MiPNet24.14 O2k-sV-Module manual|O2k-sV-Module]]'''«
+Week 21
+* '''May-20 Mo'''
+:{|
+| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
+|''' How do I determine the optimal oligomycin concentration for my assay?'''<br>
+Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity. <br>
+- »'''[https://wiki.oroboros.at/index.php/Oligomycin#Omy_titration_test Oligomycin titration test]'''« <br>
+- communicated by »[[Baglivo Eleonora]]« and »[[Schmitt Sabine]]«
+|}
 ----
-* '''Mar-24 Tu'''
+* '''May-21 Tu'''
-:::: [[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] Tuberculosis Day: '''Mitochondria as target to ''Mycobacterium tuberculosis'' infection'''
-::::::: - from the O2k-Network »[[US NC Durham Li PA]]«
-::::::* Lu X, Williams Z, Hards K, Tang J, Cheung CY, Aung HL, Wang B, Liu Z, Hu X, Lenaerts AJ, Woolhiser L, Hastings C, Zhang X, Wang Z, Rhee KY, Ding K, Zhang T, Cook GM (2018) A pyrazolo[1,5-a]pyridine inhibitor of the respiratory cytochrome bcc complex for the treatment of drug-resistant tuberculosis. ACS Infect Dis 5:239-49. - [[Lu 2018 ACS Infect Dis |»Bioblast link«]]
 ----
-* '''Mar-25 We'''
+* '''May-22 We''' - International Day for Biological Diversity
-:::: [[Image:MitoFit.jpg|left|50px|link=http://www.mitofit.org/index.php/MitoFit |MitoFit]] Preprint: '''An urgent call for research on COVID-19 and public vitamin D awareness'''
+:{|
-::::::* Brown RA, Sarkar A (2020) Vitamin D deficiency: a factor in COVID-19, progression, severity and mortality? – An urgent call for research. MitoFit Preprint Arch [[doi:10.26124/mitofit:200001]].
+| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-<br />
+|'''"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."''' <br>
-:::: [[Image:Gentle-Science_Ganesha.jpg|left|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]] '''Research findings made available via preprint servers before journal publication''' - [https://wellcome.ac.uk/press-release/sharing-research-data-and-findings-relevant-novel-coronavirus-covid-19-outbreak?sap-outbound-id=CF563AB7AA7FEDFB8731215C98A59C11790C0456&utm_source=hybris-campaign&utm_medium=email&utm_campaign=101_PBN2619_0000020211_GLS_101_aut_202003_Coronavirus_Forschung&utm_content=EN_internal_44988_20200325&mkt-key=005056A5C6311EE9999982134B93151D Sharing research data and findings relevant to the novel coronavirus (COVID-19) outbreak]
+* Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. [[Ciccone 2024 J Exp Biol|»Bioblast link«]]
+:from the O2k-Network »[[NO Tromsoe Wood SH]]«
+|}
+----
+* '''May-23 Th'''
+----
+* '''May-24 Fr'''
+----
-::::[[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]]  [[File:Blue-info.png|40px|Info: improve]] '''O<sub>2</sub> flux analysis with DatLab 7.4'''. Use our latest O<sub>2</sub> analysis template: »'''[https://www.youtube.com/watch?v=HRAGvmaK01I| O2k-Videosupport: O<sub>2</sub> flux analysis with DatLab 7.4]'''«
+'''<big>Next Week</big>'''
+Week 22
+* '''May-27 Mo'''
+:{|
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
+|'''Same experimental conditions, but different O<sub>2</sub> fluxes in the left and right chambers?'''<br>
+Check for instrumental and operational factors that could be causing O<sub>2</sub> flux differences between the O2k-chambers.
+<br>
+- »'''[[Different O2 fluxes in left and right chamber]]'''«<br>
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
+|}
 ----
-* '''Mar-26 Th'''
+* '''May-28 Tu'''
+----
+* '''May-29 We'''
+----
+* '''May-30 Th'''
+:{|
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
+|''' "Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"'''<br>
+- »'''[[MiPNet20.06_IsolationMouseHeart-mt|MiPNet20.06 Isolation mouse heart mitochondria]]'''«<br>
+- communicated by »[[Cardoso Luiza]]« and »[[Leo Elettra]]«
+|}
+----
+* '''May-31 Fr'''
 ----
-* '''Mar-27 Fr'''