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Difference between revisions of "Template:Current week ecosystem agenda"

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2022 Week 31
{{Template:Science for Ukraine}}
{{Template:Science for Ukraine}}
*'''Aug-01 Mo'''  
 
[[Image:O2k-Workshops.png|60px|link=MiPNet27.03 IOC154 Innsbruck AT]]  <big>'''[[MiPNet27.03 IOC154 Innsbruck AT|154th Oroboros O2k-Coaching Days - Advanced and NextGen-O2k]]'''</big> Aug 1-5
2024 Week 20
 
* '''May-13 Mo'''
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
|''' O2k-checklist: Get started with an O2k-experiment''' <br>
|'''Do you see fluctuations of O<sub>2</sub> slope negative following a pattern similar to the Peltier power, but without block temperature variation?'''<br>
The O2k-checklist provides an overview of all essential steps for starting an O2k-experiment.
Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O<sub>2</sub> slope negative.  
<br>
<br>
- » '''[[MiPNet20.04_O2k-checklist]]'''« <br>
- »'''[[Oxygen_flux_-_instrumental_background#Fluctuations_of_O2_slope_negative|Fluctuations of O<sub>2</sub> slope negative]]'''«<br>
- » communicated by »[[Cecatto Cristiane]]« and »[[Schmitt Sabine]]«
- communicated by »[[Cardoso Luiza]]« and »[[Grings Mateus]]«
|}
|}
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*'''Aug-02 Tu'''  
* '''May-14 Tu'''  
[[Image:O2k-Workshops.png|60px|link=MiPNet27.03 IOC154 Innsbruck AT]]  <big>'''[[MiPNet27.03 IOC154 Innsbruck AT|154th Oroboros O2k-Coaching Days - Advanced and NextGen-O2k]]'''</big> Aug 1-5
----
* '''May-15 We'''  
:{|
:{|
| width="110" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
| width="110" align="center" | [[File:Tributes to pioneers icon.jpg|left|80px|BEC Tributes to pioneers in bioenergetics|link=Tributes to pioneers in bioenergetics]]
|''' What are the substrate-uncoupler-inhibitor titration reference protocols?'''<br>
|Azzi A, Gnaiger E (2024) Tributes to pioneers in bioenergetics - wandering in the garden of memories. Bioenerg Commun 2024.3.v1. https://doi.org/10.26124/bec:2024-0003<br>
These protocols provide a common baseline for comparison of mitochondrial respiratory control in a large variety of species, tissues and cell types, mt-preparations and laboratories.
- [[Azzi_2024_BEC-Tributes |»Bioblast link«]]
<br>
- »''' [[SUIT reference protocol]]'''«
 
- communicated by »[[Cardoso Luiza HD]]« and »[[Tindle-Solomon Lisa]]«
|}
|}
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*'''Aug-03 We'''
* '''May-16 Th'''  
[[Image:O2k-Workshops.png|60px|link=MiPNet27.03 IOC154 Innsbruck AT]]  <big>'''[[MiPNet27.03 IOC154 Innsbruck AT|154th Oroboros O2k-Coaching Days - Advanced and NextGen-O2k]]'''</big> Aug 1-5
 
:{|
:{|
 
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
| width="110" align="center" | [[File:PlayVideo.jpg|left|80px|O2k-Videosupport|link=O2k-Videosupport]]
|''' "The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."'''<br>
 
- »'''[[MiPNet27.07 Sample Holder]]'''«<br>
|''' O<sub>2</sub> flux analysis with DatLab 7.4'''<br>
- communicated by »[[Willis Jaime R]]« and »[[Timon-Gomez Alba]]«
Use the O<sub>2</sub> analysis template:  <br>
 
- »'''[https://www.youtube.com/watch?v=HRAGvmaK01I%7C O2k-Videosupport: O<sub>2</sub> flux analysis with DatLab 7.4]'''«<br>
|}
|}
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* '''May-17 Fr'''
----
'''<big>Next Week</big>'''


*'''Aug-04 Th'''
  2024 Week 21
[[Image:O2k-Workshops.png|60px|link=MiPNet27.03 IOC154 Innsbruck AT]] <big>'''[[MiPNet27.03 IOC154 Innsbruck AT|154th Oroboros O2k-Coaching Days - Advanced and NextGen-O2k]]'''</big> Aug 1-5


* '''May-20 Mo'''
:{|
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
 
|''' How do I determine the optimal oligomycin concentration for my assay?'''<br>
|''' Why do we perform multiple H<sub>2</sub>O<sub>2</sub> calibrations in the Amplex® UltraRed assay?'''<br>
Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity. <br>
The sensitivity to H<sub>2</sub>O<sub>2</sub> of the fluorometric signal changes during the experiment as a function of: (''1'') experimental time and accumulating UltroxRed (similar to resorufin), (''2'') changes of the optical properties due to titrations, and (''3'') the radical scavenging capacity of the sample. Therefore, H<sub>2</sub>O<sub>2</sub> calibrations are performed before and after sample addition, and after selected titration steps.  <br>
- »'''[https://wiki.oroboros.at/index.php/Oligomycin#Omy_titration_test Oligomycin titration test]'''« <br>
 
- communicated by »[[Baglivo Eleonora]]« and »[[Schmitt Sabine]]«  
- »'''[[Amplex UltraRed]]'''« and »'''[[Komlodi_2021_BEC_AmR-O2 |Komlodi et al BEC 2021.4]]'''«<br>
|}  
|}
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* '''May-21 Tu'''
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* '''May-22 We''' - International Day for Biological Diversity
*'''Aug-05 Fr'''
[[Image:O2k-Workshops.png|60px|link=MiPNet27.03 IOC154 Innsbruck AT]]  <big>'''[[MiPNet27.03 IOC154 Innsbruck AT|154th Oroboros O2k-Coaching Days - Advanced and NextGen-O2k]]'''</big> Aug 1-5
 
:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."''' <br>
* Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. [[Ciccone 2024 J Exp Biol|»Bioblast link«]]
:from the O2k-Network »[[NO Tromsoe Wood SH]]«
|}


| width="110" align="center" | [[File:PlayVideo.jpg|left|80px|O2k-Videosupport|link=O2k-Videosupport]]
----
 
* '''May-23 Th'''  
|'''Watch these videos to get started with the Q-Module'''<br>
----
 
* '''May-24 Fr'''
- »'''[https://wiki.oroboros.at/index.php/O2k-Videosupport#Q-Module_.28NextGen-O2k_-_DatLab_7.29 O2k-Videosupport:Q-Module]'''«<br>
----
|}

Latest revision as of 08:23, 15 May 2024

Gentle Science
Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. -  »More details
A future for Ukraine is the future of its science
2024 Week 20
  • May-13 Mo
O2k-Open SupportInfo: take care Do you see fluctuations of O2 slope negative following a pattern similar to the Peltier power, but without block temperature variation?

Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O2 slope negative.
- »Fluctuations of O2 slope negative«
- communicated by »Cardoso Luiza« and »Grings Mateus«


  • May-14 Tu

  • May-15 We
BEC Tributes to pioneers in bioenergetics
Azzi A, Gnaiger E (2024) Tributes to pioneers in bioenergetics - wandering in the garden of memories. Bioenerg Commun 2024.3.v1. https://doi.org/10.26124/bec:2024-0003

- »Bioblast link«


  • May-16 Th
O2k-Open SupportInfo: improve "The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."

- »MiPNet27.07 Sample Holder«
- communicated by »Willis Jaime R« and »Timon-Gomez Alba«


  • May-17 Fr

Next Week

2024 Week 21
  • May-20 Mo
MitoPedia
How do I determine the optimal oligomycin concentration for my assay?

Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity.
- »Oligomycin titration test« 
- communicated by »Baglivo Eleonora« and »Schmitt Sabine« 


  • May-21 Tu

  • May-22 We - International Day for Biological Diversity
O2k-Publications in the MiPMap "We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."
  • Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. »Bioblast link«
from the O2k-Network »NO Tromsoe Wood SH«

  • May-23 Th

  • May-24 Fr