Difference between revisions of "Template:Current week ecosystem agenda"

Latest revision as of 16:15, 6 May 2024

Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. - »More details

-» A future for Ukraine is the future of its science

2024 Week 19

May-06 Mo

What is the effective concentration of digitonin for complete plasma membrane permeabilization?

It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane.
- »Digitonin«, »SUIT-010«
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba«

May-07 Tu

Tributes to pioneers in bioenergetics will provide a fresh look at concepts in a state of flow rather than as fixed paradigms, which tend to be forgotten or distorted without wandering in the garden of memories.

- »Tributes to pioneers in bioenergetics - wandering in the garden of memories« - a new BEC Publication Series

May-08 We

May-09 Th

"The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"

Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem »Bioblast link«

from the O2k-Network »IT Padova Morosinotto T«

May-10 Fr

Next Week

2024 Week 20

May-13 Mo

Do you see fluctuations of O₂ slope negative following a pattern similar to the Peltier power, but without block temperature variation?

Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O₂ slope negative.
- »Fluctuations of O₂ slope negative«
- communicated by »Cardoso Luiza« and »Grings Mateus«

May-14 Tu

May-15 We

May-16 Th

"The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."

- »MiPNet27.07 Sample Holder«
- communicated by »Willis Jaime R« and »Timon-Gomez Alba«

May-17 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 10
-* '''Mar-04 Mo'''
+Week 19
+* '''May-06 Mo'''
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
+| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|''' Do you observe an unstable O<sub>2</sub> slope negative following TIP2k titrations during an instrumental O<sub>2</sub> background test?  '''<br>
+|''' What is the effective concentration of digitonin for complete plasma membrane permeabilization?'''<br>
-Incorrect placement of TIP2k Filter Papers can lead to liquid accumulation and convective oxygen backdiffusion. Check for proper placement by confirming they are fully in contact with the stopper receptacle.
+It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane. <br>
-<br>
+- »'''[[Digitonin]]'''«, »'''[[SUIT-010]]'''« <br>
-- »'''[[MiPNet14.06_Instrumental_O2_background|Instrumental O<sub>2</sub> background]]'''«, »'''[https://wiki.oroboros.at/index.php/Oxygen_flux_-_instrumental_background#Unstable_O2_slope_neg._in_instrumental_background_test Unstable O<sub>2</sub> slope negative]'''«<br>
+- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«
-- communicated by »[[Grings Mateus]]« and »[[Baglivo Eleonora]]«
 |}
 ----
-* '''Mar-05 Tu'''
+* '''May-07 Tu'''
-----
-* '''Mar-06 We'''
 :{|
-| width="100" align="center" |[[File:BEC2024.1.jpg|right|150px|link=https://doi.org/10.26124/bec:2024-0001 |Gross 2024 BEC]]
+| width="110" align="center" | [[File:Tributes to pioneers icon.jpg|left|80px|BEC Tributes to pioneers in bioenergetics|link=Tributes to pioneers in bioenergetics]]
-|'''The tale of two “best-friends,” proteins BID & MTCH2, pivotal regulators of the apoptosis and metabolism programs at the mitochondria.''' <br>
+|'''Tributes to pioneers in bioenergetics''' will provide a fresh look at concepts in a state of flow rather than as fixed paradigms, which tend to be forgotten or distorted without wandering in the garden of memories.<br>
-* Gross A, (2024) A BID-MTCH2 love story: Energizing mitochondria until apoptosis do them part? Bioenerg Commun 2024.1. https://doi.org/10.26124/bec:2024-0001
+- »'''[[Tributes to pioneers in bioenergetics |Tributes to pioneers in bioenergetics - wandering in the garden of memories]]'''« - a new BEC Publication Series<br>
 |}
 ----
-* '''Mar-07 Th'''
+* '''May-08 We'''
+----
+* '''May-09 Th'''
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
+| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|''' Which medium can be used for the instrumental background test?'''<br>
+|'''"The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"''' <br>
-When using dithionite, the instrumental background test should be performed in mitochondrial respiration medium MiR05, MiR05-Kit or MiR06; or alternatively, in a strong alkaline phosphate buffer (>100 mM; >pH 8), to avoid side reactions that might interfere with the test.<br>
+* Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem [[Vera-Vives 2024 Plant Physiol Biochem|»Bioblast link«]]
-- »'''[[MiPNet14.06_Instrumental_O2_background]]'''«<br>
+:from the O2k-Network »[[IT Padova Morosinotto T]]«
-- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«
 |}
 ----
-* '''Mar-08 Fr'''
+* '''May-10 Fr'''
 ----
+'''<big>Next Week</big>'''
-'''<big>Next Week</big>'''
+Week 20
-Week 11
-* '''Mar-11 Mo'''
+* '''May-13 Mo'''
 :{|
-| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
-|''' Why is it important to perform respirometry with permeabilized muscle fibers (pfi) under a high [O<sub>2</sub>] regime? '''<br>
+|'''Do you see fluctuations of O<sub>2</sub> slope negative following a pattern similar to the Peltier power, but without block temperature variation?'''<br>
-Long diffusion distances in the pfi result in an O<sub>2</sub> diffusion limitation, which is overcome by increasing the O<sub>2</sub> partial pressure inside the chamber. Proper instrumental background correction is a must and O<sub>2</sub> dependence of ROS production should be considered.   <br>
+Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O<sub>2</sub> slope negative.
-- »'''[[Permeabilized muscle fibers]]'''«, »'''[[Oxygen flux - instrumental background]]'''« <br>
+<br>
-- communicated by »[[Grings Mateus]]« and »[[Cardoso Luiza]]«
+- »'''[[Oxygen_flux_-_instrumental_background#Fluctuations_of_O2_slope_negative|Fluctuations of O<sub>2</sub> slope negative]]'''«<br>
+- communicated by »[[Cardoso Luiza]]« and »[[Grings Mateus]]«
 |}
 ----
-* '''Mar-12 Tu'''
+* '''May-14 Tu'''
+----
+* '''May-15 We'''
 ----
-* '''Mar-13 We'''
+* '''May-16 Th'''
 :{|
-| width="100" align="center" |[[Image:BEC-logo.png|100px|link=https://www.bioenergetics-communications.org/index.php/bec |Bioenergetics Communications]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''''Rewarded'' Open Access - compensating authors..'''<br>
+|''' "The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."'''<br>
-Together with the MiP''society'', BEC is raising funds to reward the first 10 papers accepted for publication in BEC in 2024: 600 EUR to attend EBEC2024 and 2-year MiP''membership''.
+- »'''[[MiPNet27.07 Sample Holder]]'''«<br>
--»[https://whydonate.com/en/fundraising/BEC Support open access in science]«<br>
+- communicated by »[[Willis Jaime R]]« and »[[Timon-Gomez Alba]]«
-- »[https://www.bioenergetics-communications.org/index.php/bec/about/submissions Submit a manuscript]«
 |}
 ----
-* '''Mar-14 Th'''
+* '''May-17 Fr'''
-:{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''Mitochondrial ultrastructure and respiration are affected during EDL [''Extensor digitorum longus'' muscle] aging.''' <br>
-* Fernando R, Shindyapina AV, Ost M, Santesmasses D, Hu Y, Tyshkovskiy A, Yim SH, Weiss J, Gladyshev VN, Grune T, Castro JP (2023) Downregulation of mitochondrial metabolism is a driver for fast skeletal muscle loss during mouse aging.  [[Fernando 2023 Commun Biol|»Bioblast link«]]
-:from the O2k-Network »[[DE Leipzip Ost M]]«
-|}
-----
-* '''Mar-15 Fr'''
 ----