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Latest revision as of 09:37, 21 May 2024

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-» A future for Ukraine is the future of its science

2024 Week 21

May-20 Mo

How do I determine the optimal oligomycin concentration for my assay?

Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity.
- »Oligomycin titration test«
- communicated by »Baglivo Eleonora« and »Schmitt Sabine«

May-21 Tu

May-22 We - International Day for Biological Diversity

"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."

Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. »Bioblast link«

from the O2k-Network »NO Tromsoe Wood SH«

May-23 Th

May-24 Fr

Next Week

2024 Week 22

May-27 Mo

Same experimental conditions, but different O₂ fluxes in the left and right chambers?

Check for instrumental and operational factors that could be causing O₂ flux differences between the O2k-chambers.
- »Different O2 fluxes in left and right chamber«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-28 Tu

May-29 We

May-30 Th

"Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"

- »MiPNet20.06 Isolation mouse heart mitochondria«
- communicated by »Cardoso Luiza« and »Leo Elettra«

May-31 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 14
-* '''Apr-01 Mo'''  April - Parkinson's Disease Awareness Month
+Week 21
+* '''May-20 Mo'''
 :{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|'''"Although the chain of events leading to this respiration deficit is currently not understood, the findings constitute a robust assay for the progressive pathology induced by PINK1 deficiency."''' <br>
+|''' How do I determine the optimal oligomycin concentration for my assay?'''<br>
-* Gispert S, Ricciardi F, Kurz A, Azizov M, Hoepken HH, Becker D, Voos W, Leuner K, Müller WE, Kudin AP, Kunz WS, Zimmermann A, Roeper J, Wenzel D, Jendrach M, García-Arencíbia M, Fernández-Ruiz J, Huber L, Rohrer H, Barrera M, Reichert AS, Rüb U, Chen A, Nussbaum RL, Auburger G (2009) Parkinson phenotype in aged PINK1-deficient mice is accompanied by progressive mitochondrial dysfunction in absence of neurodegeneration. [[Gispert 2009 PLoS One|»Bioblast link«]]
+Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity. <br>
-:from the O2k-Network »[[US CA San Francisco Nussbaum RL]]« and »[[DE Frankfurt Eckert GP]]«
+- »'''[https://wiki.oroboros.at/index.php/Oligomycin#Omy_titration_test Oligomycin titration test]'''« <br>
-|}
+- communicated by »[[Baglivo Eleonora]]« and »[[Schmitt Sabine]]«
+|}
 ----
-* '''Apr-02 Tu'''
+* '''May-21 Tu'''
 ----
-* '''Apr-03 We'''
+* '''May-22 We''' - International Day for Biological Diversity
-----
-* '''Apr-04 Th'''
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
+| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|''' Why is it important to check the H<sub>2</sub>O<sub>2</sub> sensitivity when conducting an Amplex UltraRed (AmR) assay?
+|'''"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."''' <br>
-The sensitivity is influenced by experimental time, medium composition, added chemicals and sample, requiring multiple H<sub>2</sub>O<sub>2</sub> calibrations throughout the measurement. After the initial AmR calibration, ensure that the sensitivity is within the recommended range.'''<br>
+* Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. [[Ciccone 2024 J Exp Biol|»Bioblast link«]]
+:from the O2k-Network »[[NO Tromsoe Wood SH]]«
+|}
-- »'''[[MiPNet24.10 H2O2 flux analysis]]'''«, »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Calibration_with_H2O2 Amplex UltraRed]'''«  <br>
-- communicated by »[[Grings Mateus]]« and »[[Schmitt Sabine]]«
-|}
 ----
-* '''Apr-05 Fr'''
+* '''May-23 Th'''
+----
+* '''May-24 Fr'''
 ----
 '''<big>Next Week</big>'''
+Week 22
-Week 15
+* '''May-27 Mo'''
+:{|
-* '''Apr-08 Mo'''
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
-:::: 16:30 '''[[Gnaiger Erich|Erich Gnaiger]] presenting a [[MOSBRI Consensus Meeting 2024 Rome IT|MOSBRI Consensus Meeting]] satellite seminar at O2k-Network Lab [[IT Rome Giuffre A]]''' - ''Unlocking cell metabolism: Exploring the power of high-resolution respirometry with the NextGen-O2k''
+|'''Same experimental conditions, but different O<sub>2</sub> fluxes in the left and right chambers?'''<br>
+Check for instrumental and operational factors that could be causing O<sub>2</sub> flux differences between the O2k-chambers.
+<br>
+- »'''[[Different O2 fluxes in left and right chamber]]'''«<br>
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
+|}
+----
+* '''May-28 Tu'''
 ----
-* '''Apr-09 Tu'''
+* '''May-29 We'''
-:::: 10:00 '''[[Gnaiger Erich|Erich Gnaiger]] presenting at [[MOSBRI Consensus Meeting 2024 Rome IT]]''' - ''Quality-controlled high-resolution respirometry: bacterial and clinical applications''
 ----
-* '''Apr-10 We'''
+* '''May-30 Th'''
 :{|
-| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|''' Is fatty acid oxidation (FAO) considered as CI- or CII-driven respiration? '''<br>
+|''' "Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"'''<br>
-Neither. There is simultaneous electron transfer from FAO into the Q-junction through the electron transferring flavoprotein dehydrogenase complex (CETFDH) and Complex I. <br>
+- »'''[[MiPNet20.06_IsolationMouseHeart-mt|MiPNet20.06 Isolation mouse heart mitochondria]]'''«<br>
-- »'''[[Fatty acid oxidation]]'''«, »'''[[Gnaiger 2020 BEC MitoPathways]]'''«, »'''[[Gnaiger 2024 J Biol Chem]]'''« <br>
+- communicated by »[[Cardoso Luiza]]« and »[[Leo Elettra]]«
-- communicated by »[[Timon-Gomez Alba]]« and »[[Willis Jaime R]]«
 |}
 ----
-* '''Apr-11 Th'''
+* '''May-31 Fr'''
-World Parkinson's Disease Day
-:{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''"The markers of mitochondrial biogenesis were consistently affected in PD [Parkinson’s disease] patients group."''' <br>
-* Kamienieva I, Charzyńska A, Duszyński J, Malińska D, Szczepanowska J (2023) In search for mitochondrial biomarkers of Parkinson's disease: Findings in parkin-mutant human fibroblasts. [[Kamienieva 2023 Biochim Biophys Acta Mol Basis Dis|»Bioblast link«]]
-:from the O2k-Network »[[PL Warsaw Szewczyk A]]«
-|}
 ----
-* '''Apr-12 Fr'''