Difference between revisions of "Template:MitoPediaAlert2021"
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Using [[MitoKit-CII]] – cell permeable succinate and malonate, the [[Succinate pathway|S-pathway]] can be measured <br> | Using [[MitoKit-CII]] – cell permeable succinate and malonate, the [[Succinate pathway|S-pathway]] can be measured <br> | ||
- »'''[[SUIT-003 O2 ce D060]]'''«<br> | - »'''[[SUIT-003 O2 ce D060]]'''«<br> | ||
- communicated by »[[ | - communicated by »[[Cecatto Cristiane]]« and »[[Komlodi Timea]]« | ||
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Revision as of 08:50, 19 July 2021
- Currently Answered Questions from the »Oroboros Ecosystem Agenda«
Jul 2021
- Jul-07 We
TPP+ for potentiometric detection of mt-membrane potential (mtMP) or fluorometry with safranin?
Safranin enables measurement of the relationship between fluorescence intensity and mtMP, and if linear, the absolute mtMP can be calculated. TPP+ enables calibration for measurement of mtMP.
- »TPP+ vs safranin«
Jun 2021
- Jun-24 Th
Do you know how to select the correct fluorescence sensors and filter set?
Using different fluorescence sensors, LED and photodiode filters, the O2k-Fluo Smart-Module (or O2k-Fluo LED2-Module) enables measurement of hydrogen peroxide, ATP, mitochondrial membrane potential, calcium, and numerous other applications open for O2k-user innovation.
- »Selecting the Smart Fluo-Sensor« and »Selecting a Filter Set«
May 2021
- May-24 Mo
Immediately after switching off the light, a sharp peak of light-enhanced dark respiration is observed in algae.
- May-11 Tu
Measuring succinate pathway (S-pathway) in living cells
Using MitoKit-CII – cell permeable succinate and malonate, the S-pathway can be measured
- »SUIT-003 O2 ce D060«
- communicated by »Cecatto Cristiane« and »Komlodi Timea«
Apr 2021
Mar 2021
- Mar-16 Tu
CI inhibitors like rotenone inhibit fatty acid oxidation (FAO)-linked respiration
Inhibition of CI leads to accumulation of NADH in the mt-matrix. The resultant lack of NAD+ blocks FAO.
- »Fatty acid oxidation pathway control state«, »Mitochondrial Pathways: Chapter 5«
- communicated by »Cardoso Luiza« and »Cecatto Cristiane«
- Mar-11 Th
Internal normalization of rate – what is the difference between flux control ratio and flux control efficiency?
A flux control ratio is the ratio of oxygen flux in a specific respiratory control state, normalized for maximum flux in a common reference state. Flux control efficiency expresses the control of respiration as a fractional change of oxygen flux or flow between protocol steps. Both values vary between theoretical lower and upper limits of 0 and 1.
- »Gnaiger 2020 BEC MitoPathways - Chapter 3«
- » Posted by: »Komlodi Timea« and »Cecatto Cristiane«
- Mar-2 Tu
Measuring fatty acid oxidation (FAO)-linked respiration requires a low concentration of malate
In the absence of malate, accumulation of Acetyl-CoA inhibits FAO.
- »Fatty acid oxidation pathway control state«, »Mitochondrial Pathways: Chapter 5«
- communicated by »Cecatto Cristiane« and »Cardoso Luiza«
Jan 2021
- Jan-21 Th
Measuring ATP production with Magnesium Green – which sample preparations work?
Magnesium Green (MgG) can be used with mitochondrial preparations: isolated mitochondria, tissue homogenates, and permeabilized cells and tissues.
- »MgG« and »Publications MgG«
- communicated by »Cardoso Luiza« and »Komlodi Timea«
